Home News About Us Contact Contributors Disclaimer Privacy Policy Help FAQ

Home
Search
Quick Search
Advanced
Fulltext
Browse
Collections
Persons
My eDoc
Session History
Login
Name:
Password:
Documentation
Help
Support Wiki
Direct access to
document ID:


          Institute: MPI für Entwicklungsbiologie     Collection: Abteilung 1 - Protein Evolution (A. Lupas)     Display Documents



  history
ID: 591412.0, MPI für Entwicklungsbiologie / Abteilung 1 - Protein Evolution (A. Lupas)
Trimeric autotransporter adhesin-dependent adherence of Bartonella henselae, Bartonella quintana, and Yersinia enterocolitica to matrix components and endothelial cells under static and dynamic flow conditions
Authors:Muller, N. F.; Kaiser, P. O.; Linke, D.; Schwarz, H.; Riess, T.; Schafer, A.; Eble, J. A.; Kempf, V. A.
Date of Publication (YYYY-MM-DD):2011-07
Title of Journal:Infect Immun
Volume:79
Issue / Number:7
Start Page:2544
End Page:2553
Review Status:not specified
Audience:Not Specified
Abstract / Description:Trimeric autotransporter adhesins (TAAs) are important virulence factors of Gram-negative bacteria responsible for adherence to extracellular matrix (ECM) and host cells. Here, we analyzed three different TAAs (Bartonella adhesin A [BadA] of Bartonella henselae, variably expressed outer membrane proteins [Vomps] of Bartonella quintana, and Yersinia adhesin A [YadA] of Yersinia enterocolitica) for mediating bacterial adherence to ECM and endothelial cells. Using static (cell culture vials) and dynamic (capillary flow chambers) experimental settings, adherence of wild-type bacteria and of the respective TAA-negative strains was analyzed. Under static conditions, ECM adherence of B. henselae, B. quintana, and Y. enterocolitica was strongly dependent on the expression of their particular TAAs. YadA of Y. enterocolitica did not mediate bacterial binding to plasma or cellular fibronectin under either static or dynamic conditions. TAA-dependent host cell adherence appeared more significant under dynamic conditions although the total number of bound bacteria was diminished compared to the number under static conditions. Dynamic models expand the methodology to perform bacterial adherence experiments under more realistic, bloodstream-like conditions and allow dissection of the biological role of TAAs in ECM and host cell adherence under static and dynamic conditions.
Free Keywords:Adhesins, Bacterial/genetics/*physiology; *Bacterial Adhesion; Bacterial Outer Membrane Proteins/metabolism; Bartonella henselae/pathogenicity/*physiology; Bartonella quintana/pathogenicity/*physiology; Cell-Matrix Junctions; Cells, Cultured; Endothelial Cells/metabolism/*microbiology; Extracellular Matrix/metabolism/microbiology; Fluorescent Antibody Technique; Host-Pathogen Interactions; Humans; Microscopy, Electron, Transmission; Umbilical Veins; Virulence Factors/metabolism; Yersinia enterocolitica/pathogenicity/*physiology
External Publication Status:published
Document Type:Article
Affiliations:MPI für Entwicklungsbiologie/Abteilung 1 - Proteinevolution (Andrei Lupas)
External Affiliations:%G eng
Identifiers:ISSN:1098-5522 (Electronic) 0019-9567 (Linking) %R IAI.... [ID No:1]
URL:http://www.ncbi.nlm.nih.gov/pubmed/21536788 [ID No:2]
The scope and number of records on eDoc is subject to the collection policies defined by each institute - see "info" button in the collection browse view.