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          Institute: MPI für Entwicklungsbiologie     Collection: Abteilung 1 - Protein Evolution (A. Lupas)     Display Documents

ID: 591419.0, MPI für Entwicklungsbiologie / Abteilung 1 - Protein Evolution (A. Lupas)
Conjugal plasmid transfer in Streptomyces resembles bacterial chromosome segregation by FtsK/SpoIIIE
Authors:Vogelmann, J.; Ammelburg, M.; Finger, C.; Guezguez, J.; Linke, D.; Flotenmeyer, M.; Stierhof, Y. D.; Wohlleben, W.; Muth, G.
Date of Publication (YYYY-MM-DD):2011-06-01
Title of Journal:EMBO J
Issue / Number:11
Start Page:2246
End Page:2254
Review Status:not specified
Audience:Not Specified
Abstract / Description:Conjugation is a major route of horizontal gene transfer, the driving force in the evolution of bacterial genomes. Antibiotic producing soil bacteria of the genus Streptomyces transfer DNA in a unique process involving a single plasmid-encoded protein TraB and a double-stranded DNA molecule. However, the molecular function of TraB in directing DNA transfer from a donor into a recipient cell is unknown. Here, we show that TraB constitutes a novel conjugation system that is clearly distinguished from DNA transfer by a type IV secretion system. We demonstrate that TraB specifically recognizes and binds to repeated 8 bp motifs on the conjugative plasmid. The specific DNA recognition is mediated by helix alpha3 of the C-terminal winged-helix-turn-helix domain of TraB. We show that TraB assembles to a hexameric ring structure with a central approximately 3.1 nm channel and forms pores in lipid bilayers. Structure, sequence similarity and DNA binding characteristics of TraB indicate that TraB is derived from an FtsK-like ancestor protein, suggesting that Streptomyces adapted the FtsK/SpoIIIE chromosome segregation system to transfer DNA between two distinct Streptomyces cells.
Free Keywords:Bacterial Proteins/*metabolism; Binding Sites; Chromosome Segregation; Chromosomes, Bacterial/genetics; *Conjugation, Genetic; DNA/metabolism; DNA, Bacterial/metabolism; Evolution, Molecular; *Gene Transfer, Horizontal; Phylogeny; *Plasmids; Protein Binding; Protein Multimerization; Sequence Analysis, DNA; Sequence Homology, Amino Acid; Streptomyces coelicolor/*genetics
External Publication Status:published
Document Type:Article
Affiliations:MPI für Entwicklungsbiologie/Abteilung 1 - Proteinevolution (Andrei Lupas)
External Affiliations:%G eng
Identifiers:ISSN:1460-2075 (Electronic) 0261-4189 (Linking) %R embo... [ID No:1]
URL:http://www.ncbi.nlm.nih.gov/pubmed/21505418 [ID No:2]
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