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          Institute: MPI für medizinische Forschung     Collection: jahrbuch_2011_archival     Display Documents



ID: 597770.0, MPI für medizinische Forschung / jahrbuch_2011_archival
Crystal structure of the human thioredoxin reductase−thioredoxin complex
Translation of Title:Crystal structure of the human thioredoxin reductase−thioredoxin complex
Authors:Fritz−Wolf, Karin; Kehr, Sebastian; Stumpf, Michaela; Rahlfs, Stefan; Becker, Katja
Language:English
Date of Publication (YYYY-MM-DD):2011-07-12
Title of Journal:Nature Communications
Journal Abbrev.:Nature Communications
Volume:2
Start Page:1
End Page:8
Sequence Number of Article:383
Review Status:Peer-review
Audience:Experts Only
Intended Educational Use:No
Abstract / Description:Thioredoxin reductase 1 (TrxR1) is a homodimeric flavoprotein crucially involved in the regulation of cellular redox homeostasis, growth, and differentiation. Its importance in various diseases makes TrxR1 a highly interesting drug target. Here we present the first crystal structures of human TrxR1 in complex with its substrate thioredoxin (Trx). The carboxy−terminal redox centre is found about 20 Å apart from the amino−terminal redox centre, with no major conformational changes in TrxR or Trx. Thus, our structure confirms that the enzyme uses a flexible C−terminal arm for electron transport to its substrates, which is stabilized by a guiding bar for controlled transfer. This notion is supported by mutational analyses. Furthermore, essential residues of the interface region were characterized both structurally and functionally. The structure provides templates for future drug design, and contributes to our understanding of redox regulatory processes in mammals
External Publication Status:published
Document Type:Article
Communicated by:Wulf Kaiser
Affiliations:MPI für medizinische Forschung/Abteilung Biophysik
MPI für medizinische Forschung/Abteilung Biomolekulare Mechanismen
Identifiers:LOCALID:7713
URI:http%3A%2F%2Fwww.nature.com%2Fncomms%2Fjournal%2Fv...
URI:http%3A%2F%2Fwww.nature.com%2Fncomms%2Fjournal%2Fv...
URI:http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fpubmed%2F21750...
DOI:10.1038%2Fncomms1382
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