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          Institute: MPI für Polymerforschung     Collection: MPI Polymerforschung     Display Documents



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ID: 59784.0, MPI für Polymerforschung / MPI Polymerforschung
Secondary structure analyses of protein films on gold surfaces by circular dichroism
Authors:Shimizu, M.; Kazutoshi, K.; Morii, H.; Mitsui, K.; Knoll, Wolfgang; Nagamune, T.
Language:English
Date of Publication (YYYY-MM-DD):2003
Title of Journal:Biochemical and Biophysical Research Communications
Journal Abbrev.:Biochem. Bioph. Res. Co.
Volume:310
Issue / Number:2
Start Page:606
End Page:611
Review Status:not specified
Audience:Not Specified
Abstract / Description:In order to analyze the secondary structures of protein molecules adsorbed on gold surfaces, circular dichroism (CD) spectra were measured and the secondary structure contents of protein ultra-thin films were estimated quantitatively. A disulfide group was introduced to cytochrome b562 (cyt.b562), which is a water-soluble b-type heme protein. The cyt.b562 molecules self-assembled to form an ultra-thin protein film both on a gold substrate modified with 2,2'-dithiodiacetic acid and on a bare gold surface. CD measurements were carried out both in solution and in air, and these results were compared. The protein denaturation was partially prevented, not only in solution but also in air, by both the modification of the substrate and the introduction of the anchor group to the protein molecule. The secondary structure contents of ultra-thin protein films on flat gold surfaces were observed for the first time both in solution and in air by CD spectra. (C) 2003 Elsevier Inc. All rights reserved.
External Publication Status:published
Document Type:Article
Affiliations:MPI für Polymerforschung
External Affiliations:Univ Tokyo, Sch Engn, Dept Biochem & Chem, Bunkyo Ku, 7-3-1 Hongo, Tokyo 2238522, Japan.; Hitachi Chem Co Ltd, Ctr Res & Dev, Analyt Sci Lab, Ibaraki 3088421, Japan.; Natl Inst Adv Ind Sci & Technol, Tsukuba, Ibaraki 3068566, Japan.; Tokyo Inst Technol, Interdisciplinary Grad Sch Sci & Engn, Dept Informat Proc, Midori Ku, Yokohama, Kanagawa 2268502, Japan.
Identifiers:LOCALID:P-03-203
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