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          Institute: MPI für biophysikalische Chemie     Collection: Molekulare Biologie (Dr. Thomas M. Jovin)     Display Documents

ID: 59958.0, MPI für biophysikalische Chemie / Molekulare Biologie (Dr. Thomas M. Jovin)
Grafting a new metal ligand in the cocatalytic site of B. cereus metallo-β-lactamase: Structural flexibility without loss of activity
Authors:Rasia, R. M.; Ceolin, M.; Vila, A. J.
Date of Publication (YYYY-MM-DD):2003
Title of Journal:Protein Science
Start Page:1538
End Page:1546
Copyright:c* 2003 The Protein Society
Review Status:Peer-review
Audience:Not Specified
Abstract / Description:Metallo-(beta)-lactamases are zinc enzymes able to hydrolyze the four-membered ring of (beta)-lactam antibiotics, representing one of the latest generations of (beta)-lactamases. These enzymes belong to the zinc metallohydrolase family of the (beta)-lactamase fold. Enzymes belonging to this family have a bimetallic active site whose structure varies among different members by point substitutions of the metal ligands. In this work, we have grafted new metal ligands into the metal binding site of BcII from Bacillus cereus that mimic the ligands present in other members of this superfamily. We have characterized spectroscopically and modeled the structure of the redesigned sites, which differ substantially from the wild-type enzyme. Despite the changes introduced in the active site, the mutant enzymes retain almost full activity. These results shed some light on the possible evolutionary origin of these metalloenzymes.
Free Keywords:Metallo-(beta)-lactamase
metal site engineering
zinc enzyme
metal substitution
External Publication Status:published
Document Type:Article
Communicated by:N. N.
Affiliations:MPI für biophysikalische Chemie/Abt. Thomas Jovin / 060
External Affiliations:Molecular Biology Division, IBR (Instituto de Biolgía y Celular Rosario) CONICET (Sonsejo Nacional de Investigaciones Cientifdicas y Técnicas) and Biophsyics Section, Department of Biological Chemistry, University of Rosario, Suipacha 531, S2002LRK Rosario, Argentina; Centro Regional de Estudios Genomicos, La Plata, Argentina
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