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          Institute: MPI für Infektionsbiologie     Collection: RNA Biology     Display Documents

ID: 610069.0, MPI für Infektionsbiologie / RNA Biology
A conserved RpoS-dependent small RNA controls the synthesis of major porin OmpD
Authors:Fröhlich, Kathrin S.; Papenfort, Kai; Berger, Allison A.; Vogel, Jörg
Date of Publication (YYYY-MM-DD):2012-04
Journal Abbrev.:Nucleic Acids Res.
Issue / Number:8
Start Page:3623
End Page:3640
Copyright:This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Review Status:Peer-review
Audience:Experts Only
Abstract / Description:A remarkable feature of many small non-coding RNAs (sRNAs) of Escherichia coli and Salmonella is their accumulation in the stationary phase of bacterial growth. Several stress response regulators and sigma factors have been reported to direct the transcription of stationary phase-specific sRNAs, but a widely conserved sRNA gene that is controlled by the major stationary phase and stress sigma factor, Sigma(S) (RpoS), has remained elusive. We have studied in Salmonella the conserved SdsR sRNA, previously known as RyeB, one of the most abundant stationary phase-specific sRNAs in E. coli. Alignments of the sdsR promoter region and genetic analysis strongly suggest that this sRNA gene is selectively transcribed by Sigma(S). We show that SdsR down-regulates the synthesis of the major Salmonella porin OmpD by Hfq-dependent base pairing; SdsR thus represents the fourth sRNA to regulate this major outer membrane porin. Similar to the InvR, MicC and RybB sRNAs, SdsR recognizes the ompD mRNA in the coding sequence, suggesting that this mRNA may be primarily targeted downstream of the start codon. The SdsR-binding site in ompD was localized by 3'-RACE, an experimental approach that promises to be of use in predicting other sRNA-target interactions in bacteria.
Comment of the Author/Creator:Work in the Vogel lab is supported by the DFG (German Research Council) as part of the priority program SPP1258 Sensory and Regulatory RNAs in Prokaryotes (Grant Vo875/2-2).
External Publication Status:published
Document Type:Article
Communicated by:Beate Löhr
Affiliations:MPI für Infektionsbiologie/RNA Biology
External Affiliations:[Froehlich, Kathrin S.; Papenfort, Kai; Vogel, Joerg] Univ Wurzburg, RNA Biol Grp, Inst Mol Infect Biol, D-97080 Wurzburg, Germany
Identifiers:ISI:000303333500034 [ID No:1]
ISSN:0305-1048 [ID No:2]
DOI:10.1093/nar/gkr1156 [ID No:3]
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