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          Institute: MPI für molekulare Physiologie     Collection: Abteilung III - Physikalische Biochemie     Display Documents

ID: 611007.0, MPI für molekulare Physiologie / Abteilung III - Physikalische Biochemie
Membrane Fusion Intermediates via Directional and Full Assembly of the SNARE Complex
Authors:Hernandez, Javier M.; Stein, Alexander; Behrmann, Elmar; Riedel, Dietmar; Cypionka, Anna; Farsi, Zohreh; Walla, Peter J.; Raunser, Stefan; Jahn, Reinhard
Date of Publication (YYYY-MM-DD):2012-06-22
Title of Journal:Science
Issue / Number:6088
Start Page:1581
End Page:1584
Sequence Number of Article:1
Review Status:Peer-review
Audience:Experts Only
Intended Educational Use:No
Abstract / Description:Cellular membrane fusion is thought to proceed through intermediates including docking of apposed lipid bilayers, merging of proximal leaflets to form a hemifusion diaphragm, and fusion pore opening. A membrane-bridging four-helix complex of soluble N-ethylmaleimide-sensitive factor attachment protein receptors (SNAREs) mediates fusion. However, how assembly of the SNARE complex generates docking and other fusion intermediates is unknown. Using a cell-free reaction, we identified intermediates visually and then arrested the SNARE fusion machinery when fusion was about to begin. Partial and directional assembly of SNAREs tightly docked bilayers, but efficient fusion and an extended form of hemifusion required assembly beyond the core complex to the membrane-connecting linkers. We propose that straining of lipids at the edges of an extended docking zone initiates fusion.
External Publication Status:published
Document Type:Article
Communicated by:Jürgen Block
Affiliations:MPI für molekulare Physiologie/Abteilung III - Physikalische Biochemie/AG Dr. S. Raunser
MPI für biophysikalische Chemie
Identifiers:URL:http://dx.doi.org/10.1126/science.1221976 [Article locator with full text links]
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