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          Institute: MPI für Biophysik     Collection: Abt. Molekulare Neurogenetik     Display Documents

ID: 631610.0, MPI für Biophysik / Abt. Molekulare Neurogenetik
Promoter architecture of mouse olfactory receptor genes
Authors:Plessy, Charles; Pascarella, Giovanni; Bertin, Nicolas; Akalin, Altuna; Carrieri, Claudia; Vassalli, Anne; Lazarevic, Dejan; Severin, Jessica; Vlachouli, Christina; Simone, Roberto; Faulkner, Geoffrey J.; Kawai, Jun; Daub, Carsten O.; Zucchelli, Silvia; Hayashizaki, Yoshihide; Mombaerts, Peter; Lenhard, Boris; Gustincich, Stefano; Carninci, Piero
Date of Publication (YYYY-MM-DD):2012-03
Title of Journal:Genome Research
Journal Abbrev.:Genome Res.
Issue / Number:3
Start Page:486
End Page:497
Review Status:Peer-review
Audience:Experts Only
Abstract / Description:Odorous chemicals are detected by the mouse main olfactory epithelium (MOE) by about 1100 types of olfactory receptors (OR) expressed by olfactory sensory neurons (OSNs). Each mature OSN is thought to express only one allele of a single OR gene. Major impediments to understand the transcriptional control of OR gene expression are the lack of a proper characterization of OR transcription start sites (TSSs) and promoters, and of regulatory transcripts at OR loci. We have applied the nanoCAGE technology to profile the transcriptome and the active promoters in the MOE. nanoCAGE analysis revealed the map and architecture of promoters for 87.5% of the mouse OR genes, as well as the expression of many novel noncoding RNAs including antisense transcripts. We identified candidate transcription factors for OR gene expression and among them confirmed by chromatin immunoprecipitation the binding of TBP, EBF1 (OLF1), and MEF2A to OR promoters. Finally, we showed that a short genomic fragment flanking the major TSS of the OR gene Olfr160 (M72) can drive OSN-specific expression in transgenic mice.
External Publication Status:published
Document Type:Article
Communicated by:N. N.
Affiliations:MPI für Biophysik/Abteilung Molekulare Neurogenetik
External Affiliations:RIKEN Yokohama Institute, Omics Science Center, Yokohama, Kanagawa 230-0045, Japan;
International School for Advanced Studies, Sector of Neurobiology, Trieste 34136, Italy;
The Giovanni Armenise–Harvard Foundation Laboratory, Sector of Neurobiology, International School for Advanced Studies, Trieste 34136, Italy;
University of Bergen, Bergen Center for Computational Science–Computational Biology Unit and Sars Centre for Marine Molecular Biology Bergen, 5008, Norway;
The Rockefeller University, New York, New York 10065, USA;
Cluster in Biomedicine, Trieste 34149, Italy;
Roslin Institute and Royal (Dick) School of Veterinary Studies, The University of Edinburgh, Roslin EH25 9RG, United Kingdom;
Italian Institute of Technology–International School for Advanced Studies Unit, Trieste 34136, Italy
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