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          Institute: MPI für Entwicklungsbiologie     Collection: Abteilung 1 - Protein Evolution (A. Lupas)     Display Documents



  history
ID: 635929.0, MPI für Entwicklungsbiologie / Abteilung 1 - Protein Evolution (A. Lupas)
Structure-activity analysis of the dermcidin-derived peptide DCD-1L, an anionic antimicrobial peptide present in human sweat
Authors:Paulmann, M.; Arnold, T.; Linke, D.; Ozdirekcan, S.; Kopp, A.; Gutsmann, T.; Kalbacher, H.; Wanke, I.; Schuenemann, V. J.; Habeck, M.; Burck, J.; Ulrich, A. S.; Schittek, B.
Date of Publication (YYYY-MM-DD):2012-03-09
Title of Journal:J Biol Chem
Volume:287
Issue / Number:11
Start Page:8434
End Page:8443
Review Status:not specified
Audience:Not Specified
Abstract / Description:Dermcidin encodes the anionic amphiphilic peptide DCD-1L, which displays a broad spectrum of antimicrobial activity under conditions resembling those in human sweat. Here, we have investigated its mode of antimicrobial activity. We found that DCD-1L interacts preferentially with negatively charged bacterial phospholipids with a helix axis that is aligned flat on a lipid bilayer surface. Upon interaction with lipid bilayers DCD-1L forms oligomeric complexes that are stabilized by Zn(2+). DCD-1L is able to form ion channels in the bacterial membrane, and we propose that Zn(2+)-induced self-assembly of DCD-1L upon interaction with bacterial lipid bilayers is a prerequisite for ion channel formation. These data allow us for the first time to propose a molecular model for the antimicrobial mechanism of a naturally processed human anionic peptide that is active under the harsh conditions present in human sweat.
Free Keywords:Anti-Infective Agents/*chemistry/pharmacology; Cell Membrane/chemistry/metabolism; Humans; Peptides/*chemistry/pharmacology; Phospholipids/chemistry/metabolism; Protein Structure, Secondary; Staphylococcus aureus/growth & development/metabolism; Structure-Activity Relationship; Sweat/*chemistry/metabolism
External Publication Status:published
Document Type:Article
Affiliations:MPI für Entwicklungsbiologie/Abteilung 1 - Proteinevolution (Andrei Lupas)
MPI für Entwicklungsbiologie/Abteilungsunabhängige Arbeitsgruppen/Arbeitsgruppe Habeck
External Affiliations:%G eng
Identifiers:ISSN:1083-351X (Electronic) 0021-9258 (Linking) %R 10.1... [ID No:1]
URL:http://www.ncbi.nlm.nih.gov/pubmed/22262861 [ID No:2]
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