Home News About Us Contact Contributors Disclaimer Privacy Policy Help FAQ

Quick Search
My eDoc
Session History
Support Wiki
Direct access to
document ID:

          Institute: MPI für Entwicklungsbiologie     Collection: Abteilung 6 - Molecular Biology (D. Weigel)     Display Documents

ID: 636045.0, MPI für Entwicklungsbiologie / Abteilung 6 - Molecular Biology (D. Weigel)
Fast-forward genetics identifies plant CPL phosphatases as regulators of miRNA processing factor HYL1
Authors:Manavella, P. A.; Hagmann, J.; Ott, F.; Laubinger, S.; Franz, M.; Macek, B.; Weigel, D.
Date of Publication (YYYY-MM-DD):2012-11-09
Title of Journal:Cell
Issue / Number:4
Start Page:859
End Page:870
Review Status:not specified
Audience:Not Specified
Abstract / Description:MicroRNAs (miRNAs) are processed from primary transcripts that contain partially self-complementary foldbacks. As in animals, the core microprocessor in plants is a Dicer protein, DICER-LIKE1 (DCL1). Processing accuracy and strand selection is greatly enhanced through the RNA binding protein HYPONASTIC LEAVES 1 (HYL1) and the zinc finger protein SERRATE (SE). We have combined a luciferase-based genetic screen with whole-genome sequencing for rapid identification of new regulators of miRNA biogenesis and action. Among the first six mutants analyzed were three alleles of C-TERMINAL DOMAIN PHOSPHATASE-LIKE 1 (CPL1)/FIERY2 (FRY2). In the miRNA processing complex, SE functions as a scaffold to mediate CPL1 interaction with HYL1, which needs to be dephosphorylated for optimal activity. In the absence of CPL1, HYL1 dephosphorylation and hence accurate processing and strand selection from miRNA duplexes are compromised. Our findings thus define a new regulatory step in plant miRNA biogenesis.
Free Keywords:Arabidopsis/*metabolism; Arabidopsis Proteins/*metabolism; Calcium-Binding Proteins/metabolism; Intercellular Signaling Peptides and Proteins/metabolism; Membrane Proteins/metabolism; MicroRNAs/*metabolism; Phosphoprotein Phosphatases/*metabolism; Phosphorylation; Plant Leaves/metabolism; Plant Proteins/metabolism; *RNA Processing, Post-Transcriptional; RNA, Plant/*metabolism; RNA-Binding Proteins/*metabolism; Tobacco/metabolism; Transcription Factors/*metabolism
External Publication Status:published
Document Type:Article
Affiliations:MPI für Entwicklungsbiologie/Abteilung 6 - Molekulare Biologie (Detlef Weigel)
External Affiliations:%G eng
Identifiers:ISSN:1097-4172 (Electronic) 0092-8674 (Linking) %R 10.1... [ID No:1]
URL:http://www.ncbi.nlm.nih.gov/pubmed/23141542 [ID No:2]
The scope and number of records on eDoc is subject to the collection policies defined by each institute - see "info" button in the collection browse view.