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          Institute: MPI für medizinische Forschung     Collection: Jahrbuch 2012     Display Documents



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ID: 638102.0, MPI für medizinische Forschung / Jahrbuch 2012
Neuronal Recordings with Solid−Conductor Intracellular Nanoelectrodes (SCINEs)
Translation of Title:Neuronal Recordings with Solid−Conductor Intracellular Nanoelectrodes (SCINEs)
Authors:Angle, Matthew; Schaefer, Andreas T.
Language:English
Date of Publication (YYYY-MM-DD):2012-08-15
Title of Journal:Plosone
Journal Abbrev.:PLosONE
Volume:7
Issue / Number:8
Start Page:1
End Page:8
Sequence Number of Article:e43194
Review Status:Peer-review
Audience:Experts Only
Intended Educational Use:No
Abstract / Description:Direct electrical recording of the neuronal transmembrane potential has been crucial to our understanding of the biophysical mechanisms subserving neuronal computation. Existing intracellular recording techniques, however, limit the accuracy and duration of such measurements by changing intracellular biochemistry and/or by damaging the plasma membrane. Here we demonstrate that nanoengineered electrodes can be used to record neuronal transmembrane potentials in brain tissue without causing these physiological perturbations. Using focused ion beam milling, we have fabricated Solid−Conductor Intracellular NanoElectrodes (SCINEs), from conventional tungsten microelectrodes. SCINEs have tips that are <300 nm in diameter for several micrometers, but can be easily handled and can be inserted into brain tissue. Performing simultaneous whole−cell patch recordings, we show that SCINEs can record action potentials (APs) as well as slower, subthreshold neuronal potentials without altering cellular properties. These results show a key role for nanotechnology in the development of new electrical recording techniques in neuroscience
External Publication Status:published
Document Type:Article
Version Comment:Automatic journal name synchronization
Communicated by:Wulf Kaiser
Affiliations:MPI für medizinische Forschung/Abteilung Zellphysiologie
MPI für medizinische Forschung/Abteilung Zellphysiologie/Olfaction Web
MPI für medizinische Forschung/Max-Planck-Forschungsgruppe Neurophysiologie des Verhaltens (Andreas T. Schaefer)
Identifiers:LOCALID:7827
URI:http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fpmc%2Farticles...
URI:http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fpmc%2Farticles...
URI:http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fpmc%2Farticles...
DOI:10.1371%2Fjournal.pone.0043194
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