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          Institute: MPI für medizinische Forschung     Collection: Jahrbuch 2012     Display Documents



ID: 638116.0, MPI für medizinische Forschung / Jahrbuch 2012
High−Resolution Protein Structure Determination by Serial Femtosecond Crystallography
Translation of Title:High−Resolution Protein Structure Determination by Serial Femtosecond Crystallography
Authors:Boutet, Sébastien; Lomb, Lukas; Williams, Garth J.; Barends, Thomas; Aquila, Andrew; Doak, R. Bruce; Weierstall, Uwe; DePonte, Daniel P.; Steinbrener, Jan; Shoeman, Robert L.; Messerschmidt, Marc; Barty, Anton; White, Thomas A.; Kassemeyer, Stephan; Kirian, Richard A.; Seibert, M. Marvin; Montanez, Paul A.; Kenney, Chris; Herbst, Ryan; Hart, Philip; Pines, Jack; Haller, Gunther; Gruner, Sol M.; Philipp, Hugh T.; Tate, Mark W.; Hromalik, Marianne; Koerner, Lucas J.; van Bakel, Niels; Morse, John; Ghonsalves, Wilfred; Arnlund, David; Bogan, Michael J.; Caleman, Carl; Fromme, Raimund; Hampton, Christina Y.; Hunter, Mark S.; Johansson, Linda; Katona, Gergely; Kupitz, Christopher; Liang, Mengning; Martin, Andrew V.; Nass, Karol; Redecke, Lars; Stellato, Francesco; Timneanu, Nicusor; Wang, Dingjie; Zatsepin, Nadia A. A.; Schafer, Donald; Defever, James; Neutze, Richard; Fromme, Petra; Spence, John C. H.; Chapman, Henry N.; Schlichting, Ilme
Language:English
Date of Publication (YYYY-MM-DD):2012-07-20
Title of Journal:Science
Journal Abbrev.:Science
Volume:337
Issue / Number:6092
Start Page:362
End Page:364
Review Status:Peer-review
Audience:Experts Only
Intended Educational Use:No
Abstract / Description:Structure determination of proteins and other macromolecules has historically required the growth of high−quality crystals sufficiently large to diffract x−rays efficiently while withstanding radiation damage. We applied serial femtosecond crystallography (SFX) using an x−ray free−electron laser (XFEL) to obtain high−resolution structural information from microcrystals (<1×1×3 μm(3)) of the well−characterized model protein lysozyme. The agreement with synchrotron data demonstrates the immediate relevance of SFX for analyzing the structure of the large group of difficult−to−crystallize molecules
External Publication Status:published
Document Type:Article
Communicated by:Wulf Kaiser
Affiliations:MPI für medizinische Forschung/Abteilung Biophysik
MPI für medizinische Forschung/Abteilung Biomolekulare Mechanismen
MPI für medizinische Forschung/Abteilung Biomolekulare Mechanismen/Molecular chaperones
MPI für medizinische Forschung/Abteilung Biomolekulare Mechanismen/Heme and Flavin Enzymes
MPI für medizinische Forschung/Abteilung Biomolekulare Mechanismen/Analytical Protein Biochemistry
MPI für medizinische Forschung/Abteilung Biomolekulare Mechanismen/Coherent diffractive imaging
Identifiers:LOCALID:7813
URI:http%3A%2F%2Fwww.sciencemag.org%2Fcontent%2Fearly%...
URI:http%3A%2F%2Fwww.sciencemag.org%2Fcontent%2Fearly%...
URI:http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fpubmed%2F22653...
DOI:10.1126%2Fscience.1217737
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