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          Institute: MPI für medizinische Forschung     Collection: Jahrbuch 2012     Display Documents



ID: 638130.0, MPI für medizinische Forschung / Jahrbuch 2012
Single−particle structure determination by correlations of snapshot X−ray diffraction patterns
Translation of Title:Single−particle structure determination by correlations of snapshot X−ray diffraction patterns
Authors:Starodub, Dmitri; Aquila, Andrew; Bajt, Saša; Barthelmess, Miriam; Barty, Anton; Bostedt, Christoph; Bozek, John D.; Coppola, Nicola; Doak, R. Bruce; Epp, Sascha W.; Erk, Benjamin; Foucar, Lutz; Gumprecht, Lars; Hampton, Christina Y.; Hartmann, Andreas; Hartmann, Robert; Holl, Peter; Kassemeyer, Stephan; Kimmel, Nils; Laksmono, Hartawan; Liang, Mengning; Loh, Ne−Te Duane; Lomb, Lukas; Martin, Andrew V.; Nass, Karol; Reich, Christian; Rolles, Daniel; Rudek, Benedikt; Rudenko, Artem; Schulz, Joachim; Shoeman, Robert L.; Sierra, Raymond G.; Soltau, Heike; Steinbrener, Jan; Stellato, Francesco; Stern, Stephan; Weidenspointner, Georg; Frank, Matthias; Ullrich, Joachim; Strüder, Lothar; Schlichting, Ilme; Chapman, Henry N.; Spence, John C. H.; Bogan, Michael J.
Language:English
Date of Publication (YYYY-MM-DD):2012-12-11
Title of Journal:Nature Communications
Journal Abbrev.:Nature Communications
Volume:3
Start Page:1
End Page:7
Sequence Number of Article:1276
Review Status:Peer-review
Audience:Experts Only
Intended Educational Use:No
Abstract / Description:Diffractive imaging with free−electron lasers allows structure determination from ensembles of weakly scattering identical nanoparticles. The ultra−short, ultra−bright X−ray pulses provide snapshots of the randomly oriented particles frozen in time, and terminate before the onset of structural damage. As signal strength diminishes for small particles, the synthesis of a three−dimensional diffraction volume requires simultaneous involvement of all data. Here we report the first application of a three−dimensional spatial frequency correlation analysis to carry out this synthesis from noisy single−particle femtosecond X−ray diffraction patterns of nearly identical samples in random and unknown orientations, collected at the Linac Coherent Light Source. Our demonstration uses unsupported test particles created via aerosol self−assembly, and composed of two polystyrene spheres of equal diameter. The correlation analysis avoids the need for orientation determination entirely. This method may be applied to the structural determination of biological macromolecules in solution
External Publication Status:published
Document Type:Article
Communicated by:Wulf Kaiser
Affiliations:MPI für medizinische Forschung/Abteilung Biophysik
MPI für medizinische Forschung/Abteilung Biomolekulare Mechanismen
MPI für medizinische Forschung/Abteilung Biomolekulare Mechanismen/Heme and Flavin Enzymes
MPI für medizinische Forschung/Abteilung Biomolekulare Mechanismen/Analytical Protein Biochemistry
MPI für medizinische Forschung/Abteilung Biomolekulare Mechanismen/Coherent diffractive imaging
Identifiers:LOCALID:7840
URI:http%3A%2F%2Fwww.nature.com%2Fncomms%2Fjournal%2Fv...
URI:http%3A%2F%2Fwww.nature.com%2Fncomms%2Fjournal%2Fv...
URI:http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fpubmed%2F23232...
DOI:10.1038%2Fncomms2288
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