Home News About Us Contact Contributors Disclaimer Privacy Policy Help FAQ

Home
Search
Quick Search
Advanced
Fulltext
Browse
Collections
Persons
My eDoc
Session History
Login
Name:
Password:
Documentation
Help
Support Wiki
Direct access to
document ID:


          Institute: MPI für medizinische Forschung     Collection: Jahrbuch 2012     Display Documents



ID: 638137.0, MPI für medizinische Forschung / Jahrbuch 2012
Charting Monosynaptic Connectivity Maps by Two−Color Light−Sheet
Fluorescence Microscopy
Translation of Title:Charting Monosynaptic Connectivity Maps by Two−Color Light−Sheet
Fluorescence Microscopy
Authors:Niedworok, Christian J.; Schwarz, Inna; Ledderose, Julia; Giese, Günter; Conzelmann, Karl−Klaus; Schwarz, Martin K.
Language:English
Date of Publication (YYYY-MM-DD):2012-11-29
Title of Journal:Cell Reports
Journal Abbrev.:Cell Reports
Volume:2
Issue / Number:5
Start Page:1375
End Page:1386
Review Status:Peer-review
Audience:Experts Only
Intended Educational Use:No
Abstract / Description:Cellular resolution three−dimensional (3D) visualization of defined, fluorescently labeled long−range neuronal networks in the uncut adult mouse brain has been elusive. Here, a virus−based strategy is described that allowed fluorescent labeling of centrifugally projecting neuronal populations in the ventral forebrain and their directly, monosynaptically connected bulbar interneurons upon a single stereotaxic injection into select neuronal populations. Implementation of improved tissue clearing combined with light−sheet fluorescence microscopy permitted imaging of the resulting connectivity maps in a single whole−brain scan. Subsequent 3D reconstructions revealed the exact distribution of the diverse neuronal ensembles monosynaptically connected with distinct bulbar interneuron populations. Moreover, rehydratation of brains after light−sheet fluorescence imaging enabled the immunohistochemical identification of synaptically connected neurons. Thus, this study describes a method for identifying monosynaptic connectivity maps from distinct, virally labeled neuronal populations that helps in better understanding of information flow in neural systems
External Publication Status:published
Document Type:Article
Communicated by:Wulf Kaiser
Affiliations:MPI für medizinische Forschung/Abteilung Molekulare Neurobiologie
MPI für medizinische Forschung/Abteilung Biomedizinische Optik
MPI für medizinische Forschung/Abteilung Molekulare Neurobiologie/Gruppe Rolf Sprengel
MPI für medizinische Forschung/Abteilung Molekulare Neurobiologie/Gruppe Martin Schwarz
Identifiers:LOCALID:7829
URI:http%3A%2F%2Fdownload.cell.com%2Fcell-reports%2Fpd...
URI:http%3A%2F%2Fwww.cell.com%2Fcell-reports%2Fabstrac...
URI:http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fpubmed%2F23142...
DOI:10.1016%2Fj.celrep.2012.10.008
Full Text:
Sorry, no privileges
The scope and number of records on eDoc is subject to the collection policies defined by each institute - see "info" button in the collection browse view.