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          Institute: MPI für medizinische Forschung     Collection: Jahrbuch 2012     Display Documents

ID: 638139.0, MPI für medizinische Forschung / Jahrbuch 2012
Time−resolved protein nanocrystallography
using an X−ray free−electron laser
Translation of Title:Time−resolved protein nanocrystallography
using an X−ray free−electron laser
Authors:Aquila, Andrew; Hunter, Mark S.; Doak, R. Bruce; Kirian, Richard A.; Fromme, Petra; White, Thomas A.; Andreasson, Jakob; Arnlund, David; Bajt, Saša; Barends, Thomas; Barthelmess, Miriam; Bogan, Michael J.; Bostedt, Christoph; Bottin, Hervé; Bozek, John D.; Caleman, Carl; Coppola, Nicola; Davidsson, Jan; DePonte, Daniel P.; Elser, Veit; Epp, Sascha W.; Erk, Benjamin; Fleckenstein, Holger; Foucar, Lutz; Frank, Matthias; Fromme, Raimund; Graafsma, Heinz; Grotjohann, Ingo; Gumprecht, Lars; Hajdu, Janos; Hampton, Christina Y.; Hartmann, Andreas; Hartmann, Robert; Hau−Riege, Stefan P.; Hauser, Günter; Hirsemann, Helmut; Holl, Peter; Holton, James M.; Hömke, André; Johansson, Linda; Kimmel, Nils; Kassemeyer, Stephan; Krasniqi, Faton; Kühnel, Kai−Uwe; Liang, Mengning; Lomb, Lukas; Malmerberg, Erik; Marchesini, Stefano; Martin, Andrew V.; Maia, Filipe R.N.C.; Messerschmidt, Marc; Nass, Karol; Reich, Christian; Neutze, Richard; Rolles, Daniel; Rudek, Benedikt; Rudenko, Artem; Schlichting, Ilme; Schmidt, Carlo; Schmidt, Kevin E.; Schulz, Joachim; Seibert, M. Marvin; Shoeman, Robert L.; Sierra, Raymond G.; Soltau, Heike; Starodub, Dmitri; Stellato, Francesco; Stern, Stephan; Strüder, Lothar; Timneanu, Nicusor; Ullrich, Joachim; Wang, Xiaoyu; Williams, Garth J.; Weidenspointner, Georg; Weierstall, Uwe; Wunderer, Cornelia B.; Barty, Anton; Spence, John C. H.; Chapman, Henry N.
Date of Publication (YYYY-MM-DD):2012-01-30
Title of Journal:Optics Express
Journal Abbrev.:Optics Express
Issue / Number:3
Start Page:2706
End Page:2716
Review Status:Peer-review
Audience:Experts Only
Intended Educational Use:No
Abstract / Description:We demonstrate the use of an X−ray free electron laser
synchronized with an optical pump laser to obtain X−ray diffraction
snapshots from the photoactivated states of large membrane protein
complexes in the form of nanocrystals flowing in a liquid jet. Light−induced
changes of Photosystem I−Ferredoxin co−crystals were observed at time
delays of 5 to 10 μs after excitation. The result correlates with the
microsecond kinetics of electron transfer from Photosystem I to ferredoxin.
The undocking process that follows the electron transfer leads to large
rearrangements in the crystals that will terminally lead to the disintegration
of the crystals. We describe the experimental setup and obtain the first timeresolved
femtosecond serial X−ray crystallography results from an irreversible photo−chemical reaction at the Linac Coherent Light Source.
This technique opens the door to time−resolved structural studies of reaction
dynamics in biological systems
External Publication Status:published
Document Type:Article
Communicated by:Wulf Kaiser
Affiliations:MPI für medizinische Forschung/Abteilung Biophysik
MPI für medizinische Forschung/Abteilung Biomolekulare Mechanismen
MPI für medizinische Forschung/Abteilung Biomolekulare Mechanismen/Molecular chaperones
MPI für medizinische Forschung/Abteilung Biomolekulare Mechanismen/Heme and Flavin Enzymes
MPI für medizinische Forschung/Abteilung Biomolekulare Mechanismen/Analytical Protein Biochemistry
MPI für medizinische Forschung/Abteilung Biomolekulare Mechanismen/Coherent diffractive imaging
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