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| ID:
638139.0,
MPI für medizinische Forschung / Jahrbuch 2012 |
Time−resolved protein nanocrystallography
using an X−ray free−electron laser |
| Translation of Title: | Time−resolved protein nanocrystallography
using an X−ray free−electron laser | | Authors: | Aquila, Andrew; Hunter, Mark S.; Doak, R. Bruce; Kirian, Richard A.; Fromme, Petra; White, Thomas A.; Andreasson, Jakob; Arnlund, David; Bajt, Saša; Barends, Thomas; Barthelmess, Miriam; Bogan, Michael J.; Bostedt, Christoph; Bottin, Hervé; Bozek, John D.; Caleman, Carl; Coppola, Nicola; Davidsson, Jan; DePonte, Daniel P.; Elser, Veit; Epp, Sascha W.; Erk, Benjamin; Fleckenstein, Holger; Foucar, Lutz; Frank, Matthias; Fromme, Raimund; Graafsma, Heinz; Grotjohann, Ingo; Gumprecht, Lars; Hajdu, Janos; Hampton, Christina Y.; Hartmann, Andreas; Hartmann, Robert; Hau−Riege, Stefan P.; Hauser, Günter; Hirsemann, Helmut; Holl, Peter; Holton, James M.; Hömke, André; Johansson, Linda; Kimmel, Nils; Kassemeyer, Stephan; Krasniqi, Faton; Kühnel, Kai−Uwe; Liang, Mengning; Lomb, Lukas; Malmerberg, Erik; Marchesini, Stefano; Martin, Andrew V.; Maia, Filipe R.N.C.; Messerschmidt, Marc; Nass, Karol; Reich, Christian; Neutze, Richard; Rolles, Daniel; Rudek, Benedikt; Rudenko, Artem; Schlichting, Ilme; Schmidt, Carlo; Schmidt, Kevin E.; Schulz, Joachim; Seibert, M. Marvin; Shoeman, Robert L.; Sierra, Raymond G.; Soltau, Heike; Starodub, Dmitri; Stellato, Francesco; Stern, Stephan; Strüder, Lothar; Timneanu, Nicusor; Ullrich, Joachim; Wang, Xiaoyu; Williams, Garth J.; Weidenspointner, Georg; Weierstall, Uwe; Wunderer, Cornelia B.; Barty, Anton; Spence, John C. H.; Chapman, Henry N. | | Language: | English | | Date of Publication (YYYY-MM-DD): | 2012-01-30 | | Title of Journal: | Optics Express | | Journal Abbrev.: | Optics Express | | Volume: | 20 | | Issue / Number: | 3 | | Start Page: | 2706 | | End Page: | 2716 | | Review Status: | Peer-review | | Audience: | Experts Only | | Intended Educational Use: | No | | Abstract / Description: | We demonstrate the use of an X−ray free electron laser
synchronized with an optical pump laser to obtain X−ray diffraction
snapshots from the photoactivated states of large membrane protein
complexes in the form of nanocrystals flowing in a liquid jet. Light−induced
changes of Photosystem I−Ferredoxin co−crystals were observed at time
delays of 5 to 10 μs after excitation. The result correlates with the
microsecond kinetics of electron transfer from Photosystem I to ferredoxin.
The undocking process that follows the electron transfer leads to large
rearrangements in the crystals that will terminally lead to the disintegration
of the crystals. We describe the experimental setup and obtain the first timeresolved
femtosecond serial X−ray crystallography results from an irreversible photo−chemical reaction at the Linac Coherent Light Source.
This technique opens the door to time−resolved structural studies of reaction
dynamics in biological systems | | External Publication Status: | published | | Document Type: | Article |
| Communicated by: | Wulf Kaiser | | Affiliations: | MPI für medizinische Forschung/Abteilung Biophysik
MPI für medizinische Forschung/Abteilung Biomolekulare Mechanismen
MPI für medizinische Forschung/Abteilung Biomolekulare Mechanismen/Molecular chaperones
MPI für medizinische Forschung/Abteilung Biomolekulare Mechanismen/Heme and Flavin Enzymes
MPI für medizinische Forschung/Abteilung Biomolekulare Mechanismen/Analytical Protein Biochemistry
MPI für medizinische Forschung/Abteilung Biomolekulare Mechanismen/Coherent diffractive imaging
| | Identifiers: | LOCALID:7760
URI:http%3A%2F%2Fwww.opticsinfobase.org%2Foe%2Fviewmed...
URI:http%3A%2F%2Fwww.opticsinfobase.org%2Foe%2Fviewmed...
URI:http%3A%2F%2Fwww.opticsinfobase.org%2Foe%2Fabstrac...
DOI:10.1364%2FOE.20.002706 |
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