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          Institute: MPI für medizinische Forschung     Collection: Jahrbuch 2012     Display Documents



ID: 638147.0, MPI für medizinische Forschung / Jahrbuch 2012
RNA Editing of the IQ Domain in Cav1.3 Channels Modulates Their Ca2+−Dependent Inactivation
Translation of Title:RNA Editing of the IQ Domain in Cav1.3 Channels Modulates Their Ca2+−Dependent Inactivation
Authors:Huang, Hua; Tan, Bao Zhen; Shen, Yiru; Tao, Jin; Jiang, Fengli; Sung, Ying Ying; Ng, Choon Keow; Raida, Manfred; Köhr, Georg; Higuchi, Miyoko; Fatemi−Shariatpanahi, Hadi; Harden, Bradley; Yue, David T.; Soong, Tuck Wah
Language:English
Date of Publication (YYYY-MM-DD):2012-01-26
Title of Journal:Neuron
Journal Abbrev.:Neuron
Volume:73
Issue / Number:2
Start Page:304
End Page:316
Review Status:Peer-review
Audience:Experts Only
Intended Educational Use:No
Abstract / Description:Adenosine−to−inosine RNA editing is crucial for generating molecular diversity, and serves to regulate protein function through recoding of genomic information. Here, we discover editing within CaV1.3 Ca2+ channels, renown for low−voltage Ca2+−influx and neuronal pacemaking. Significantly, editing occurs within the channel's IQ domain, a calmodulin−binding site mediating inhibitory Ca2+−feedback (CDI) on channels. The editing turns out to require RNA adenosine deaminase ADAR2, whose variable activity could underlie a spatially diverse pattern of CaV1.3 editing seen across the brain. Edited CaV1.3 protein is detected both in brain tissue and within the surface membrane of primary neurons. Functionally, edited CaV1.3 channels exhibit strong reduction of CDI; in particular, neurons within the suprachiasmatic nucleus show diminished CDI, with higher frequencies of repetitive action−potential and calcium−spike activity, in wild−type versus ADAR2 knockout mice. Our study reveals a mechanism for fine−tuning CaV1.3 channel properties in CNS, which likely impacts a broad spectrum of neurobiological functions
External Publication Status:published
Document Type:Article
Communicated by:Wulf Kaiser
Affiliations:MPI für medizinische Forschung/Abteilung Molekulare Neurobiologie
MPI für medizinische Forschung/Abteilung Molekulare Neurobiologie/Gruppe Georg Köhr
Identifiers:LOCALID:7736
URI:http%3A%2F%2Fpdn.sciencedirect.com%2Fscience%3F_ob...
URI:http%3A%2F%2Fwww.sciencedirect.com%2Fscience%2Fart...
URI:http%3A%2F%2Fwww.cell.com%2Fneuron%2Fabstract%2FS0...
DOI:10.1016%2Fj.neuron.2011.11.022
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