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          Institute: MPI für molekulare Zellbiologie und Genetik     Collection: MPI-CBG Publications 2012-arch     Display Documents



ID: 645248.0, MPI für molekulare Zellbiologie und Genetik / MPI-CBG Publications 2012-arch
Functional repurposing revealed by comparing S. pombe and S. cerevisiae genetic interactions.
Authors:Frost, Adam; Elgort, Marc G; Brandman, Onn; Ives, Clinton; Collins, Sean R.; Miller-Vedam, Lakshmi; Weibezahn, Jimena; Hein, Marco Y; Poser, Ina; Mann, Matthias; Hyman, Anthony A.; Weissman, Jonathan S.
Date of Publication (YYYY-MM-DD):2012
Title of Journal:Cell
Volume:149
Issue / Number:6
Start Page:1339
End Page:1352
Copyright:not available
Audience:Experts Only
Intended Educational Use:No
Abstract / Description:We present a genetic interaction map of pairwise measures including ∼40% of nonessential S. pombe genes. By comparing interaction maps for fission and budding yeast, we confirmed widespread conservation of genetic relationships within and between complexes and pathways. However, we identified an important subset of orthologous complexes that have undergone functional "repurposing": the evolution of divergent functions and partnerships. We validated three functional repurposing events in S. pombe and mammalian cells and discovered that (1) two lumenal sensors of misfolded ER proteins, the kinase/nuclease Ire1 and the glucosyltransferase Gpt1, act together to mount an ER stress response; (2) ESCRT factors regulate spindle-pole-body duplication; and (3) a membrane-protein phosphatase and kinase complex, the STRIPAK complex, bridges the cis-Golgi, the centrosome, and the outer nuclear membrane to direct mitotic progression. Each discovery opens new areas of inquiry and-together-have implications for model organism-based research and the evolution of genetic systems.
External Publication Status:published
Document Type:Article
Communicated by:nn
Affiliations:MPI für molekulare Zellbiologie und Genetik
Identifiers:LOCALID:4942
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