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          Institute: MPI für molekulare Zellbiologie und Genetik     Collection: MPI-CBG Publications 2012-arch     Display Documents



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ID: 645270.0, MPI für molekulare Zellbiologie und Genetik / MPI-CBG Publications 2012-arch
The highly processive kinesin-8, Kip3, switches microtubule protofilaments with a bias towards the left
Authors:Bormuth, Volker; Nitzsche, Bert; Ruhnow, Felix; Mitra, Aniruddha; Storch, Marko; Rammner, Burkhard; Howard, Jonathon; Diez, Stefan
Date of Publication (YYYY-MM-DD):2012
Title of Journal:Biophysical Journal
Volume:103
Issue / Number:1 (Letter)
Start Page:4
End Page:6
Copyright:not available
Audience:Experts Only
Intended Educational Use:No
Abstract / Description:Kinesin-1 motor proteins walk parallel to the protofilament axes of microtubules as they step from one tubulin dimer to the next. Is protofilament tracking an inherent property of processive kinesin motors, like kinesin-1, and what are the structural determinants underlying protofilament tracking? To address these questions, we investigated the tracking properties of the processive kinesin-8, Kip3. Using in vitro gliding motility assays, we found that Kip3 rotates microtubules counterclockwise around their longitudinal axes with periodicities of ∼1 μm. These rotations indicate that the motors switch protofilaments with a bias toward the left. Molecular modeling suggests 1), that the protofilament switching may be due to kinesin-8 having a longer neck linker than kinesin-1, and 2), that the leftward bias is due the asymmetric geometry of the motor neck linker complex.
External Publication Status:published
Document Type:Article
Version Comment:Automatic journal name synchronization
Communicated by:nn
Affiliations:MPI für molekulare Zellbiologie und Genetik
Identifiers:LOCALID:4793
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