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          Institute: MPI für molekulare Zellbiologie und Genetik     Collection: MPI-CBG Publications 2012-arch     Display Documents



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ID: 645285.0, MPI für molekulare Zellbiologie und Genetik / MPI-CBG Publications 2012-arch
Hierarchical assembly of the eggshell and permeability barrier in C. elegans.
Authors:Olson, Sara K; Greenan, Garrett; Desai, Arshad; Müller-Reichert, Thomas; Oegema, Karen
Date of Publication (YYYY-MM-DD):2012
Title of Journal:The Journal of Cell Biology
Volume:198
Issue / Number:4
Start Page:731
End Page:748
Copyright:not available
Audience:Experts Only
Intended Educational Use:No
Abstract / Description:In metazoans, fertilization triggers the assembly of an extracellular coat that constitutes the interface between the embryo and its environment. In nematodes, this coat is the eggshell, which provides mechanical rigidity, prevents polyspermy, and is impermeable to small molecules. Using immunoelectron microscopy, we found that the Caenorhabditis elegans eggshell was composed of an outer vitelline layer, a middle chitin layer, and an inner layer containing chondroitin proteoglycans. The switch between the chitin and proteoglycan layers was achieved by internalization of chitin synthase coincident with exocytosis of proteoglycan-containing cortical granules. Inner layer assembly did not make the zygote impermeable as previously proposed. Instead, correlative light and electron microscopy demonstrated that the permeability barrier was a distinct envelope that formed in a separate step that required fatty acid synthesis, the sugar-modifying enzyme PERM-1, and the acyl chain transfer enzyme DGTR-1. These findings delineate the hierarchy of eggshell assembly and define key molecular mechanisms at each step.
External Publication Status:published
Document Type:Article
Version Comment:Automatic journal name synchronization
Communicated by:nn
Affiliations:MPI für molekulare Zellbiologie und Genetik
Identifiers:LOCALID:5252
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