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          Institute: MPI für molekulare Zellbiologie und Genetik     Collection: MPI-CBG Publications 2012-arch     Display Documents



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ID: 645293.0, MPI für molekulare Zellbiologie und Genetik / MPI-CBG Publications 2012-arch
One-step purification of assembly-competent tubulin from diverse eukaryotic sources.
Authors:Widlund, Per; Podolski, Marija; Reber, Simone; Alper, Joshua; Storch, Marko; Hyman, Anthony; Howard, Jonathon; Drechsel, David N.
Date of Publication (YYYY-MM-DD):2012
Title of Journal:Molecular Biology of the Cell
Volume:23
Issue / Number:22
Start Page:4393
End Page:4401
Copyright:not available
Audience:Experts Only
Intended Educational Use:No
Abstract / Description:We have developed a protocol that allows rapid and efficient purification of native, active tubulin from a variety of species and tissue sources by affinity chromatography. The affinity matrix comprises a bacterially expressed, recombinant protein, the TOG1/2 domains from Saccharomyces cerevisiae Stu2, covalently coupled to a Sepharose support. The resin has a high capacity to specifically bind tubulin from clarified crude cell extracts, and, after washing, highly purified tubulin can be eluted under mild conditions. The eluted tubulin is fully functional and can be efficiently assembled into microtubules. The method eliminates the need to use heterologous systems for the study of microtubule-associated proteins and motor proteins, which has been a major issue in microtubule-related research.
External Publication Status:published
Document Type:Article
Version Comment:Automatic journal name synchronization
Communicated by:nn
Affiliations:MPI für molekulare Zellbiologie und Genetik
Identifiers:LOCALID:5233
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