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          Institute: MPI für molekulare Zellbiologie und Genetik     Collection: MPI-CBG Publications 2012-arch     Display Documents



ID: 645309.0, MPI für molekulare Zellbiologie und Genetik / MPI-CBG Publications 2012-arch
A Genome-Scale Resource for In Vivo Tag-Based Protein Function Exploration in C. elegans.
Authors:Sarov, Mihail; Murray, John I; Schanze, Kristin; Pozniakovski, Andrei; Niu, Wei; Angermann, Karolin; Hasse, Susanne; Rupprecht, Michaela; Vinis, Elisabeth; Tinney, Matthew; Preston, Elicia A.; Zinke, Andrea; Enst, Susanne; Teichgraber, Tina; Janette, Judith; Reis, Kadri; Janosch, Stephan; Schloissnig, Siegfried; Ejsmont, Radoslaw K; Slightam, Cindie; Xu, Xiao; Kim, Stuart K; Reinke, Valerie; Stewart, A Francis; Snyder, Michael; Waterston, Robert H; Hyman, Anthony A.
Date of Publication (YYYY-MM-DD):2012
Title of Journal:Cell
Volume:150
Issue / Number:4
Start Page:855
End Page:866
Copyright:not available
Audience:Experts Only
Intended Educational Use:No
Abstract / Description:Understanding the in vivo dynamics of protein localization and their physical interactions is important for many problems in biology. To enable systematic protein function interrogation in a multicellular context, we built a genome-scale transgenic platform for in vivo expression of fluorescent- and affinity-tagged proteins in Caenorhabditis elegans under endogenous cis regulatory control. The platform combines computer-assisted transgene design, massively parallel DNA engineering, and next-generation sequencing to generate a resource of 14,637 genomic DNA transgenes, which covers 73% of the proteome. The multipurpose tag used allows any protein of interest to be localized in vivo or affinity purified using standard tag-based assays. We illustrate the utility of the resource by systematic chromatin immunopurification and automated 4D imaging, which produced detailed DNA binding and cell/tissue distribution maps for key transcription factor proteins.
External Publication Status:published
Document Type:Article
Communicated by:nn
Affiliations:MPI für molekulare Zellbiologie und Genetik
Identifiers:LOCALID:4944
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