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          Institute: MPI für molekulare Biomedizin     Collection: Yearbook 2013     Display Documents



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ID: 648337.0, MPI für molekulare Biomedizin / Yearbook 2013
Isolation of novel multipotent neural crest-derived stem cells from adult human inferior turbinate
Authors:Hauser, S.; Widera, D.; Qunneis, F.; Muller, J.; Zander, C.; Greiner, J.; Strauss, C.; Luningschror, P.; Heimann, P.; Schwarze, H.; Ebmeyer, J.; Sudhoff, H.; Arauzo-Bravo, M. J.; Greber, B.; Zaehres, H.; Scholer, H.; Kaltschmidt, C.; Kaltschmidt, B.
Date of Publication (YYYY-MM-DD):2012-03-20
Title of Journal:Stem Cells and Development
Volume:21
Issue / Number:5
Start Page:742
End Page:756
Review Status:Internal review
Audience:Not Specified
Abstract / Description:Adult human neural crest-derived stem cells (NCSCs) are of extraordinary high plasticity and promising candidates for the use in regenerative medicine. Here we describe for the first time a novel neural crest-derived stem cell population within the respiratory epithelium of human adult inferior turbinate. In contrast to superior and middle turbinates, high amounts of source material could be isolated from human inferior turbinates. Using minimally-invasive surgery methods isolation is efficient even in older patients. Within their endogenous niche, inferior turbinate stem cells (ITSCs) expressed high levels of nestin, p75(NTR), and S100. Immunoelectron microscopy using anti-p75 antibodies displayed that ITSCs are of glial origin and closely related to nonmyelinating Schwann cells. Cultivated ITSCs were positive for nestin and S100 and the neural crest markers Slug and SOX10. Whole genome microarray analysis showed pronounced differences to human ES cells in respect to pluripotency markers OCT4, SOX2, LIN28, and NANOG, whereas expression of WDR5, KLF4, and c-MYC was nearly similar. ITSCs were able to differentiate into cells with neuro-ectodermal and mesodermal phenotype. Additionally ITSCs are able to survive and perform neural crest typical chain migration in vivo when transplanted into chicken embryos. However ITSCs do not form teratomas in severe combined immunodeficient mice. Finally, we developed a separation strategy based on magnetic cell sorting of p75(NTR) positive ITSCs that formed larger neurospheres and proliferated faster than p75(NTR) negative ITSCs. Taken together our study describes a novel, readily accessible source of multipotent human NCSCs for potential cell-replacement therapy.
Free Keywords:Adult; Animals; Blotting, Western; Cell Differentiation/genetics; Cell Movement; Cell Proliferation; Cells, Cultured; Chick Embryo; Gene Expression Profiling; HEK293 Cells; Humans; Intermediate Filament Proteins/genetics/metabolism; Mice; Mice, SCID; Microscopy, Electron, Scanning; Microscopy, Immunoelectron; Multipotent Stem Cells/*cytology/metabolism/ultrastructure; Nerve Tissue Proteins/genetics/metabolism; Neural Crest/*cytology/metabolism; Neural Stem Cells/*cytology/metabolism/ultrastructure; Octamer Transcription Factor-3/genetics/metabolism; Pluripotent Stem Cells/cytology/metabolism; Reverse Transcriptase Polymerase Chain Reaction; SOXB1 Transcription Factors/genetics/metabolism; Stem Cell Transplantation/methods; Transplantation, Heterologous; Turbinates/*cytology/metabolism
External Publication Status:published
Document Type:Article
Communicated by:keuker
Affiliations:MPI für molekulare Biomedizin
External Affiliations:%G eng
Identifiers:ISSN:1557-8534 (Electronic) 1547-3287 (Linking) %R 10.1... [ID No:1]
URL:http://www.ncbi.nlm.nih.gov/pubmed/22128806 [ID No:2]
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