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          Institute: MPI für molekulare Biomedizin     Collection: Yearbook 2013     Display Documents



  history
ID: 648338.0, MPI für molekulare Biomedizin / Yearbook 2013
Direct visualization of cell division using high-resolution imaging of M-phase of the cell cycle
Authors:Hesse, M.; Raulf, A.; Pilz, G. A.; Haberlandt, C.; Klein, A. M.; Jabs, R.; Zaehres, H.; Fugemann, C. J.; Zimmermann, K.; Trebicka, J.; Welz, A.; Pfeifer, A.; Roll, W.; Kotlikoff, M. I.; Steinhauser, C.; Gotz, M.; Schöler, H. R.; Fleischmann, B. K.
Date of Publication (YYYY-MM-DD):2012
Title of Journal:Nat Commun
Volume:3
Start Page:1076
Review Status:Internal review
Audience:Not Specified
Abstract / Description:Current approaches to monitor and quantify cell division in live cells, and reliably distinguish between acytokinesis and endoreduplication, are limited and complicate determination of stem cell pool identities. Here we overcome these limitations by generating an in vivo reporter system using the scaffolding protein anillin fused to enhanced green fluorescent protein, to provide high spatiotemporal resolution of mitotic phase. This approach visualizes cytokinesis and midbody formation as hallmarks of expansion of stem and somatic cells, and enables distinction from cell cycle variations. High-resolution microscopy in embryonic heart and brain tissues of enhanced green fluorescent protein-anillin transgenic mice allows live monitoring of cell division and quantitation of cell cycle kinetics. Analysis of cell division in hearts post injury shows that border zone cardiomyocytes in the infarct respond with increasing ploidy, but not cell division. Thus, the enhanced green fluorescent protein-anillin system enables monitoring and measurement of cell division in vivo and markedly simplifies in vitro analysis in fixed cells.
Free Keywords:Animals; Brain/embryology/metabolism; Cell Cycle/genetics/*physiology; Cell Division/genetics/*physiology; Contractile Proteins/genetics/metabolism; Embryonic Stem Cells/cytology/metabolism; Flow Cytometry; Green Fluorescent Proteins/genetics/metabolism; Heart/embryology; Humans; Induced Pluripotent Stem Cells/cytology/metabolism; Mice; Mitosis/genetics/*physiology; Myocardial Infarction/genetics/metabolism
External Publication Status:published
Document Type:Article
Communicated by:keuker
Affiliations:MPI für molekulare Biomedizin
External Affiliations:%G eng
Identifiers:ISSN:2041-1723 (Electronic) 2041-1723 (Linking) %R 10.1... [ID No:1]
URL:http://www.ncbi.nlm.nih.gov/pubmed/23011130 [ID No:2]
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