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          Institute: MPI für Chemie     Collection: Publikationen MPI für Chemie     Display Documents



ID: 675660.0, MPI für Chemie / Publikationen MPI für Chemie
Determination of nitration degrees for the birch pollen allergen Bet v 1
Authors:Selzle, K.; Ackaert, C.; Kampf, C. J.; Kunert, A. T.; Duschl, A.; Oostingh, G. J.; Pöschl, U.
Language:English
Date of Publication (YYYY-MM-DD):2013-11
Title of Journal:Analytical and Bioanalytical Chemistry
Volume:405
Issue / Number:27
Start Page:8945
End Page:8949
Review Status:Internal review
Audience:Experts Only
Abstract / Description:Nitration of tyrosine residues in the major birch pollen allergen Bet v 1 may alter the allergenic potential of the protein. The kinetics and mechanism of the nitration reaction, however, have not yet been well characterized. To facilitate further investigations, an efficient method to quantify the nitration degree (ND) of small samples of Bet v 1 is required. Here, we present a suitable method of high-performance liquid chromatography coupled to a diode array detector (HPLC-DAD) that can be photometrically calibrated using the amino acids tyrosine (Tyr) and nitrotyrosine (NTyr) without the need for nitrated protein standards. The new method is efficient and in agreement with alternative methods based on hydrolysis and amino acid analysis of tetranitromethane (TNM)-nitrated Bet v 1 standards as well as samples from nitration experiments with peroxynitrite. The results confirm the applicability of the new method for the investigation of the reaction kinetics and mechanism of protein nitration.
External Publication Status:published
Document Type:Article
Version Comment:Automatic journal name synchronization
Communicated by:N. N.
Affiliations:MPI für Chemie
Identifiers:ISI:000325823600028 [ID No:1]
ISSN:1618-2642 [ID No:2]
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