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          Institute: MPI für bioanorganische Chemie     Collection: MPI für bioanorganische Chemie     Display Documents



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ID: 677356.0, MPI für bioanorganische Chemie / MPI für bioanorganische Chemie
Spontaneous activation of [FeFe]-hydrogenases by an inorganic [2Fe] active site mimic
Authors:Esselborn, Julian; Lambertz, Camilla; Adamska-Venkatesh, Agnieszka; Simmons, Trevor; Berggren, Gustav; Nothl, Jens; Siebel, Judith F.; Hemschemeier, Anja; Artero, Vincent; Reijerse, Edward J.; Fontecave, Marc; Lubitz, Wolfgang; Happe, Thomas
Language:English
Date of Publication (YYYY-MM-DD):2013
Title of Journal:Nature Chemical Biology
Journal Abbrev.:Nat. Chem. Biol.
Volume:9
Issue / Number:10
Start Page:607
End Page:609
Review Status:Peer-review
Audience:Experts Only
Abstract / Description:Hydrogenases catalyze the formation of hydrogen. The cofactor ('H-cluster') of [FeFe]-hydrogenases consists of a [4Fe-4S] cluster bridged to a unique [2Fe] subcluster whose biosynthesis in vivo requires hydrogenase-specific maturases. Here we show that a chemical mimic of the [2Fe] subcluster can reconstitute apo-hydrogenase to full activity, independent of helper proteins. The assembled H-cluster is virtually indistinguishable from the native cofactor. This procedure will be a powerful tool for developing new artificial H-2-producing catalysts.
External Publication Status:published
Document Type:Article
Communicated by:N. N.
Affiliations:MPI für bioanorganische Chemie
External Affiliations:[Esselborn, J.; Lambertz, Camilla; Nothl, J.; Hemschemeier, A.; Happe, T.] Ruhr Univ Bochum, Fak Biol & Biotechnol, AG Photobiotechnol, Bochum, Germany.
[Simmons', T.; Berggren, G.; Artero, V.; Fontecave, M.] Univ Grenoble 1, CNRS, Lab Chim & Biol Metaux, Commissariat Energie Atom & Energies Alternat, Grenoble, France.
[Fontecave, M.] Coll France, F-75231 Paris, France.
[Berggren, G.]Stockholm Univ, Dept Biochem & Biophys, S-10691 Stockholm, Sweden.
Identifiers:ISI:000325041800006 [ID No:1]
ISSN:1552-4450 [ID No:2]
DOI:10.1038/nchembio.1311 [ID No:3]
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