Home News About Us Contact Contributors Disclaimer Privacy Policy Help FAQ

Home
Search
Quick Search
Advanced
Fulltext
Browse
Collections
Persons
My eDoc
Session History
Login
Name:
Password:
Documentation
Help
Support Wiki
Direct access to
document ID:


          Institute: MPI für medizinische Forschung     Collection: Jahbruch 2014_archival     Display Documents



ID: 681450.0, MPI für medizinische Forschung / Jahbruch 2014_archival
Serial femtosecond X−ray diffraction of 30S ribosomal subunit microcrystals in liquid suspension at ambient temperature using an X−ray free−electron laser
Translation of Title:Serial femtosecond X−ray diffraction of 30S ribosomal subunit microcrystals in liquid suspension at ambient temperature using an X−ray free−electron laser
Authors:Demirci, Hasan; Sierra, Raymond G.; Laksmono, Hartawan; Shoeman, Robert; Botha, Sabine; Barends, Thomas; Nass, Karol; Schlichting, Ilme; Doak, R. Bruce; Gati, Cornelius; Williams, Garth J.; Boutet, Sébastien; Messerschmidt, Marc; Jogl, Gerwald; Dahlberg, Albert E.; Gregory, Steven T.; Bogan, Michael J.
Language:English
Date of Publication (YYYY-MM-DD):2013-09-01
Title of Journal:Acta Chrystalographica Section F
Journal Abbrev.:Acta Chrystalographica Section F
Volume:69
Issue / Number:9
Start Page:1066
End Page:1069
Review Status:Peer-review
Audience:Experts Only
Intended Educational Use:No
Abstract / Description:High−resolution ribosome structures determined by X−ray crystallography have
provided important insights into the mechanism of translation. Such studies
have thus far relied on large ribosome crystals kept at cryogenic temperatures
to reduce radiation damage. Here, the application of serial femtosecond X−ray
crystallography (SFX) using an X−ray free−electron laser (XFEL) to obtain
diffraction data from ribosome microcrystals in liquid suspension at ambient
temperature is described. 30S ribosomal subunit microcrystals diffracted to
beyond 6 A
̊
resolution, demonstrating the feasibility of using SFX for ribosome
structural studies. The ability to collect diffraction data at near−physiological
temperatures promises to provide fundamental insights into the structural
dynamics of the ribosome and its functional complexes
Free Keywords:30S ribosomal subunit; serial femtosecond X−ray crystallography; X−ray free−electron laser; ribosome
External Publication Status:published
Document Type:Article
Communicated by:wkaiser
Affiliations:MPI für medizinische Forschung/Abteilung Biophysik
MPI für medizinische Forschung/Abteilung Biomolekulare Mechanismen
MPI für medizinische Forschung/Abteilung Biomolekulare Mechanismen/Molecular chaperones
MPI für medizinische Forschung/Abteilung Biomolekulare Mechanismen/Coherent diffractive imaging
Identifiers:LOCALID:7920
URI:http%3A%2F%2Fjournals.iucr.org%2Ff%2Fissues%2F2013...
URI:http%3A%2F%2Fjournals.iucr.org%2Ff%2Fissues%2F2013...
URI:http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fpubmed%2F23989...
DOI:10.1107%2FS174430911302099X
Full Text:
Sorry, no privileges
The scope and number of records on eDoc is subject to the collection policies defined by each institute - see "info" button in the collection browse view.