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          Institute: MPI für medizinische Forschung     Collection: Jahbruch 2014_archival     Display Documents



ID: 681463.0, MPI für medizinische Forschung / Jahbruch 2014_archival
A ternary AppA−PpsR−DNA complex mediates light regulation of photosynthesis−related gene expression
Translation of Title:A ternary AppA−PpsR−DNA complex mediates light regulation of photosynthesis−related gene expression
Authors:Winkler, Andreas; Heintz, Udo; Lindner, Robert; Reinstein, Jochen; Shoeman, Robert L.; Schlichting, Ilme
Language:English
Date of Publication (YYYY-MM-DD):2013-06-02
Title of Journal:Nature Structural and Molecular Biology
Journal Abbrev.:Nat. Struct. Mol. Biol.
Volume:20
Issue / Number:7
Start Page:859
End Page:867
Review Status:Peer-review
Audience:Experts Only
Intended Educational Use:No
Abstract / Description:The anoxygenic phototrophic bacterium Rhodobacter sphaeroides uses different energy sources, depending on environmental conditions including aerobic respiration or, in the absence of oxygen, photosynthesis. Photosynthetic genes are repressed at high oxygen tension, but at intermediate levels their partial expression prepares the bacterium for using light energy. Illumination, however, enhances repression under semiaerobic conditions. Here, we describe molecular details of two proteins mediating oxygen and light control of photosynthesis−gene expression: the light−sensing antirepressor AppA and the transcriptional repressor PpsR. Our crystal structures of both proteins and their complex and hydrogen/deuterium−exchange data show that light activation of AppA−PpsR2 affects the PpsR effector region within the complex. DNA binding studies demonstrate the formation of a light−sensitive ternary AppA−PpsR−DNA complex. We discuss implications of these results for regulation by light and oxygen, highlighting new insights into blue light−mediated signal transduction
External Publication Status:published
Document Type:Article
Communicated by:wkaiser
Affiliations:MPI für medizinische Forschung/Abteilung Biophysik
MPI für medizinische Forschung/Abteilung Biomolekulare Mechanismen
MPI für medizinische Forschung/Abteilung Biomolekulare Mechanismen/Molecular chaperones
MPI für medizinische Forschung/Abteilung Biomolekulare Mechanismen/Analytical Protein Biochemistry
MPI für medizinische Forschung/Abteilung Biomolekulare Mechanismen/Coherent diffractive imaging
Identifiers:LOCALID:7907
URI:http%3A%2F%2Fwww.nature.com%2Fnsmb%2Fjournal%2Fvao...
URI:http%3A%2F%2Fwww.nature.com%2Fnsmb%2Fjournal%2Fvao...
URI:http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fpubmed%2F23728...
DOI:10.1038%2Fnsmb.2597
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