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          Institute: MPI für medizinische Forschung     Collection: Jahbruch 2014_archival     Display Documents



ID: 681480.0, MPI für medizinische Forschung / Jahbruch 2014_archival
Staining and embedding the whole mouse brain for electron microscopy
Translation of Title:Staining and embedding the whole mouse brain for electron microscopy
Authors:Mikula, Shawn; Binding, Jonas; Denk, Winfried
Language:English
Date of Publication (YYYY-MM-DD):2013-12-01
Title of Journal:Nature Methods
Journal Abbrev.:Nature Methods
Volume:9
Issue / Number:12
Start Page:1198
End Page:1201
Review Status:Peer-review
Audience:Experts Only
Intended Educational Use:No
Abstract / Description:The development of methods for imaging large contiguous volumes with the electron microscope could allow the complete mapping of a whole mouse brain at the single−axon level. We developed a method based on prolonged immersion that enables staining and embedding of the entire mouse brain with uniform myelin staining and a moderate preservation of the tissue's ultrastructure. We tested the ability to follow myelinated axons using serial block−face electron microscopy
External Publication Status:published
Document Type:Article
Communicated by:wkaiser
Affiliations:MPI für medizinische Forschung/Abteilung Biomedizinische Optik
Identifiers:LOCALID:7854
URI:http%3A%2F%2Fwww.nature.com%2Fnmeth%2Fjournal%2Fv9...
URI:http%3A%2F%2Fwww.nature.com%2Fnmeth%2Fjournal%2Fv9...
URI:http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fpubmed%2F23085...
DOI:10.1038%2Fnmeth.2213
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