Home News About Us Contact Contributors Disclaimer Privacy Policy Help FAQ

Home
Search
Quick Search
Advanced
Fulltext
Browse
Collections
Persons
My eDoc
Session History
Login
Name:
Password:
Documentation
Help
Support Wiki
Direct access to
document ID:


          Institute: MPI für Herz- und Lungenforschung (W. G. Kerckhoff Institut)     Collection: Yearbook_2014     Display Documents



ID: 682145.0, MPI für Herz- und Lungenforschung (W. G. Kerckhoff Institut) / Yearbook_2014
Transcriptome analysis of newt lens regeneration reveals distinct gradients in gene expression patterns
Authors:Sousounis, K.; Looso, M.; Maki, N.; Ivester, C. J.; Braun, T.; Tsonis, P. A.
Date of Publication (YYYY-MM-DD):2013
Journal Abbrev.:PLoS One
Volume:8
Issue / Number:4
Start Page:e61445
Audience:Not Specified
Abstract / Description:Regeneration of the lens in newts is quite a unique process. The lens is removed in its entirety and regeneration ensues from the pigment epithelial cells of the dorsal iris via transdifferentiation. The same type of cells from the ventral iris are not capable of regenerating a lens. It is, thus, expected that differences between dorsal and ventral iris during the process of regeneration might provide important clues pertaining to the mechanism of regeneration. In this paper, we employed next generation RNA-seq to determine gene expression patterns during lens regeneration in Notophthalmus viridescens. The expression of more than 38,000 transcripts was compared between dorsal and ventral iris. Although very few genes were found to be dorsal- or ventral-specific, certain groups of genes were up-regulated specifically in the dorsal iris. These genes are involved in cell cycle, gene regulation, cytoskeleton and immune response. In addition, the expression of six highly regulated genes, TBX5, FGF10, UNC5B, VAX2, NR2F5, and NTN1, was verified using qRT-PCR. These graded gene expression patterns provide insight into the mechanism of lens regeneration, the markers that are specific to dorsal or ventral iris, and layout a map for future studies in the field.
Free Keywords:Animals; Gene Expression Profiling; Iris/metabolism/physiology; Lens, Crystalline/*metabolism/physiology; Notophthalmus viridescens/genetics/physiology; Real-Time Polymerase Chain Reaction; Regeneration/genetics/physiology; Salamandridae/*genetics/*physiology; Transcriptome/*genetics
External Publication Status:published
Document Type:Article
Communicated by:Svea Hümmer
Affiliations:MPI für physiologische und klinische Forschung
Identifiers:ISSN:1932-6203 (Electronic) 1932-6203 (Linking) %R 10.1371/journal.pone.0061445
URL:http://www.ncbi.nlm.nih.gov/pubmed/23613853
The scope and number of records on eDoc is subject to the collection policies defined by each institute - see "info" button in the collection browse view.