Home News About Us Contact Contributors Disclaimer Privacy Policy Help FAQ

Home
Search
Quick Search
Advanced
Fulltext
Browse
Collections
Persons
My eDoc
Session History
Login
Name:
Password:
Documentation
Help
Support Wiki
Direct access to
document ID:


          Institute: MPI für Herz- und Lungenforschung (W. G. Kerckhoff Institut)     Collection: Yearbook_2014     Display Documents



ID: 682166.0, MPI für Herz- und Lungenforschung (W. G. Kerckhoff Institut) / Yearbook_2014
Bone marrow transplantation improves the outcome of Atm-deficient mice through the migration of ATM-competent cells
Authors:Pietzner, J.; Baer, P. C.; Duecker, R. P.; Merscher, M. B.; Satzger-Prodinger, C.; Bechmann, I.; Wietelmann, A.; Del Turco, D.; Doering, C.; Kuci, S.; Bader, P.; Schirmer, S.; Zielen, S.; Schubert, R.
Date of Publication (YYYY-MM-DD):2013-02-01
Journal Abbrev.:Hum Mol Genet
Volume:22
Issue / Number:3
Start Page:493
End Page:507
Audience:Not Specified
Abstract / Description:Ataxia telangiectasia (A-T) is a highly pleiotropic disorder. Patients suffer from progressive neurodegeneration, severe bronchial complications, immunodeficiency, hypersensitivity to radiotherapy and elevated risk of malignancies. Leukemia and lymphoma, along with lung failure, are the main causes of morbidity and mortality in A-T patients. At present, no effective therapy for A-T exists. One promising therapeutic approach is bone marrow transplantation (BMT) that is already used as a curative therapy for other genomic instability syndromes. We used an established clinically relevant non-myeloablative host-conditioning regimen and transplanted green fluorescent protein (GFP)-expressing ataxia telangiectasia mutated (ATM)-competent bone marrow-derived cells (BMDCs) into Atm-deficient mice. GFP expression allowed tracking of the potential migration of the cells into the tissues of recipient animals. Donor BMDCs migrated into the bone marrow, blood, thymus, spleen and lung tissue of Atm-deficient mice showing an ATM-competent phenotype. BMT inhibited thymic lymphomas, normalized T-lymphocyte populations, improved weight gain and rearing activity of Atm-deficient mice. In contrast, no GFP(+) cells were found in the cerebellum or cerebrum, and we detected decreased size index in MRI imaging of the cerebellum in 8-month-old transplanted Atm-deficient mice in comparison to wild-type mice. The repopulation with ATM-competent BMDCs is associated with a prolonged lifespan and significantly improved the phenotype of Atm-deficient mice.
Free Keywords:Animals; Ataxia Telangiectasia/genetics/pathology/*therapy; Ataxia Telangiectasia Mutated Proteins; Blood-Brain Barrier/metabolism; Blotting, Western; *Bone Marrow Transplantation; CD4-Positive T-Lymphocytes/metabolism; CD8-Positive T-Lymphocytes/metabolism; Cell Cycle Proteins/*genetics/metabolism; *Cell Movement; Chimerism; DNA-Binding Proteins/*genetics/metabolism; Disease Models, Animal; Genotype; Green Fluorescent Proteins/genetics/metabolism; Lung/cytology/metabolism; Magnetic Resonance Imaging; Mice; Mice, Transgenic; Peripheral Blood Stem Cell Transplantation; Phenotype; Phosphorylation; Protein-Serine-Threonine Kinases/*genetics/metabolism; Spleen/metabolism; Thymus Gland/metabolism; Tumor Suppressor Proteins/*genetics/metabolism
External Publication Status:published
Document Type:Article
Communicated by:Svea Hümmer
Affiliations:MPI für physiologische und klinische Forschung
Identifiers:ISSN:1460-2083 (Electronic) 0964-6906 (Linking) %R 10.1093/hmg/dds448
URL:http://www.ncbi.nlm.nih.gov/pubmed/23100326
The scope and number of records on eDoc is subject to the collection policies defined by each institute - see "info" button in the collection browse view.