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          Institute: MPI für Herz- und Lungenforschung (W. G. Kerckhoff Institut)     Collection: Yearbook_2014     Display Documents



ID: 682169.0, MPI für Herz- und Lungenforschung (W. G. Kerckhoff Institut) / Yearbook_2014
Jmjd3 controls mesodermal and cardiovascular differentiation of embryonic stem cells
Authors:Ohtani, K.; Zhao, C.; Dobreva, G.; Manavski, Y.; Kluge, B.; Braun, T.; Rieger, M. A.; Zeiher, A. M.; Dimmeler, S.
Date of Publication (YYYY-MM-DD):2013-09-13
Journal Abbrev.:Circ Res
Volume:113
Issue / Number:7
Start Page:856
End Page:862
Audience:Not Specified
Abstract / Description:RATIONALE: The developmental role of the H3K27 demethylases Jmjd3, especially its epigenetic regulation at target genes in response to upstream developmental signaling, is unclear. OBJECTIVE: To determine the role of Jmjd3 during mesoderm and cardiovascular lineage commitment. METHODS AND RESULTS: Ablation of Jmjd3 in mouse embryonic stem cells does not affect the maintenance of pluripotency and self-renewal but compromised mesoderm and subsequent endothelial and cardiac differentiation. Jmjd3 reduces H3K27me3 marks at the Brachyury promoter and facilitates the recruitment of beta-catenin, which is critical for Wnt signal-induced mesoderm differentiation. CONCLUSIONS: These data demonstrate that Jmjd3 is required for mesoderm differentiation and cardiovascular lineage commitment.
Free Keywords:Animals; *Cell Differentiation; Cell Line; Cell Lineage; Embryonic Stem Cells/cytology/*metabolism; Endothelial Cells/cytology; Endothelium, Vascular/*cytology; Fetal Proteins/genetics/metabolism; Jumonji Domain-Containing Histone Demethylases/genetics/*metabolism; Mesoderm/*cytology/metabolism; Mice; Mutation; Myocytes, Cardiac/*cytology; Promoter Regions, Genetic; T-Box Domain Proteins/genetics/metabolism; Wnt Signaling Pathway; beta Catenin/metabolism
External Publication Status:published
Document Type:Article
Communicated by:Svea Hümmer
Affiliations:MPI für physiologische und klinische Forschung
Identifiers:ISSN:1524-4571 (Electronic) 0009-7330 (Linking) %R 10.1161/CIRCRESAHA.113.302035
URL:http://www.ncbi.nlm.nih.gov/pubmed/23856522
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