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          Institute: MPI für Herz- und Lungenforschung (W. G. Kerckhoff Institut)     Collection: Yearbook_2014     Display Documents

ID: 682194.0, MPI für Herz- und Lungenforschung (W. G. Kerckhoff Institut) / Yearbook_2014
Specification of hepatopancreas progenitors in zebrafish by hnf1ba and wnt2bb
Authors:Lancman, J. J.; Zvenigorodsky, N.; Gates, K. P.; Zhang, D.; Solomon, K.; Humphrey, R. K.; Kuo, T.; Setiawan, L.; Verkade, H.; Chi, Y. I.; Jhala, U. S.; Wright, C. V.; Stainier, D. Y.; Dong, P. D.
Date of Publication (YYYY-MM-DD):2013-07
Journal Abbrev.:Development
Issue / Number:13
Start Page:2669
End Page:2679
Audience:Not Specified
Abstract / Description:Although the liver and ventral pancreas are thought to arise from a common multipotent progenitor pool, it is unclear whether these progenitors of the hepatopancreas system are specified by a common genetic mechanism. Efforts to determine the role of Hnf1b and Wnt signaling in this crucial process have been confounded by a combination of factors, including a narrow time frame for hepatopancreas specification, functional redundancy among Wnt ligands, and pleiotropic defects caused by either severe loss of Wnt signaling or Hnf1b function. Using a novel hypomorphic hnf1ba zebrafish mutant that exhibits pancreas hypoplasia, as observed in HNF1B monogenic diabetes, we show that hnf1ba plays essential roles in regulating beta-cell number and pancreas specification, distinct from its function in regulating pancreas size and liver specification, respectively. By combining Hnf1ba partial loss of function with conditional loss of Wnt signaling, we uncover a crucial developmental window when these pathways synergize to specify the entire ventrally derived hepatopancreas progenitor population. Furthermore, our in vivo genetic studies demonstrate that hnf1ba generates a permissive domain for Wnt signaling activity in the foregut endoderm. Collectively, our findings provide a new model for HNF1B function, yield insight into pancreas and beta-cell development, and suggest a new mechanism for hepatopancreatic specification.
Free Keywords:Animals; Animals, Genetically Modified; Cell Differentiation/genetics/physiology; Hepatocyte Nuclear Factor 1-beta/genetics/*metabolism; Hepatopancreas/*cytology/*metabolism; Signal Transduction/genetics/physiology; Stem Cells/*cytology/*metabolism; Wnt Proteins/genetics/*metabolism; Zebrafish; Zebrafish Proteins/genetics/*metabolism
External Publication Status:published
Document Type:Article
Communicated by:Svea Hümmer
Affiliations:MPI für physiologische und klinische Forschung
Identifiers:ISSN:1477-9129 (Electronic) 0950-1991 (Linking) %R 10.1242/dev.090993
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