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          Institute: MPI für Herz- und Lungenforschung (W. G. Kerckhoff Institut)     Collection: Yearbook_2014     Display Documents



ID: 682198.0, MPI für Herz- und Lungenforschung (W. G. Kerckhoff Institut) / Yearbook_2014
Stable isotope labeling in zebrafish allows in vivo monitoring of cardiac morphogenesis
Authors:Konzer, A.; Ruhs, A.; Braun, H.; Jungblut, B.; Braun, T.; Kruger, M.
Date of Publication (YYYY-MM-DD):2013-06
Journal Abbrev.:Mol Cell Proteomics
Volume:12
Issue / Number:6
Start Page:1502
End Page:1512
Audience:Not Specified
Abstract / Description:Quantitative proteomics is an important tool to study biological processes, but so far it has been challenging to apply to zebrafish. Here, we describe a large scale quantitative analysis of the zebrafish proteome using a combination of stable isotope labeling and liquid chromatography-mass spectrometry (LC-MS). Proteins derived from the fully labeled fish were used as a standard to quantify changes during embryonic heart development. LC-MS-assisted analysis of the proteome of activated leukocyte cell adhesion molecule zebrafish morphants revealed a down-regulation of components of the network required for cell adhesion and maintenance of cell shape as well as secondary changes due to arrest of cellular differentiation. Quantitative proteomics in zebrafish using the stable isotope-labeling technique provides an unprecedented resource to study developmental processes in zebrafish.
Free Keywords:Animals; Cell Adhesion; Cell Adhesion Molecules/*genetics/metabolism; Cell Shape; Chromatography, Liquid; Fish Proteins/*genetics/metabolism; Gene Expression Profiling; *Gene Expression Regulation, Developmental; Heart/*embryology; Isotope Labeling; Leukocytes/cytology/metabolism; Mass Spectrometry; Morphogenesis/*genetics; Proteome/*genetics/metabolism; Zebrafish/embryology/*genetics/metabolism
External Publication Status:published
Document Type:Article
Communicated by:Svea Hümmer
Affiliations:MPI für physiologische und klinische Forschung
Identifiers:ISSN:1535-9484 (Electronic) 1535-9476 (Linking) %R 10.1074/mcp.M111.015594
URL:http://www.ncbi.nlm.nih.gov/pubmed/23412571
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