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          Institute: MPI für Herz- und Lungenforschung (W. G. Kerckhoff Institut)     Collection: Yearbook_2014     Display Documents



ID: 682208.0, MPI für Herz- und Lungenforschung (W. G. Kerckhoff Institut) / Yearbook_2014
Endotoxin induces proliferation of NSCLC in vitro and in vivo: role of COX-2 and EGFR activation
Authors:Hattar, K.; Savai, R.; Subtil, F. S.; Wilhelm, J.; Schmall, A.; Lang, D. S.; Goldmann, T.; Eul, B.; Dahlem, G.; Fink, L.; Schermuly, R. T.; Banat, G. A.; Sibelius, U.; Grimminger, F.; Vollmer, E.; Seeger, W.; Grandel, U.
Date of Publication (YYYY-MM-DD):2013-02
Journal Abbrev.:Cancer Immunol Immunother
Volume:62
Issue / Number:2
Start Page:309
End Page:320
Audience:Not Specified
Abstract / Description:Lung cancer is frequently complicated by pulmonary infections which may impair prognosis of this disease. Therefore, we investigated the effect of bacterial lipopolysaccharides (LPS) on tumor proliferation in vitro in the non-small cell lung cancer (NSCLC) cell line A549, ex vivo in a tissue culture model using human NSCLC specimens and in vivo in the A549 adenocarcinoma mouse model. LPS induced a time- and dose-dependent increase in proliferation of A549 cells as quantified by MTS activity and cell counting. In parallel, an increased expression of the proliferation marker Ki-67 and cyclooxygenase (COX)-2 was detected both in A549 cells and in ex vivo human NSCLC tissue. Large amounts of COX-2-derived prostaglandin (PG)E(2) were secreted from LPS-stimulated A549 cells. Pharmacological interventions revealed that the proliferative effect of LPS was dependent on CD14 and Toll-like receptor (TLR)4. Moreover, blocking of the epidermal growth factor receptor (EGFR) also decreased LPS-induced proliferation of A549 cells. Inhibition of COX-2 activity in A549 cells severely attenuated both PGE(2) release and proliferation in response to LPS. Synthesis of PGE(2) was also reduced by inhibiting CD14, TLR4 and EGFR in A549 cells. The proliferative effect of LPS on A549 cells could be reproduced in the A549 adenocarcinoma mouse model with enhancement of tumor growth and Ki-67 expression in implanted tumors. In summary, LPS induces proliferation of NSCLC cells in vitro, ex vivo in human NSCLC specimen and in vivo in a mouse model of NSCLC. Pulmonary infection may thus directly induce tumor progression in NSCLC.
Free Keywords:Animals; Antigens, CD14/metabolism; Carcinoma, Non-Small-Cell Lung/metabolism/*pathology; Cell Line, Tumor; Cell Proliferation/drug effects; Cyclooxygenase 1/*metabolism; Cyclooxygenase 2/*metabolism; Cyclooxygenase Inhibitors/pharmacology; Dinoprostone/analysis/secretion; Disease Models, Animal; Humans; Indomethacin/pharmacology; Ki-67 Antigen/biosynthesis; Lipopolysaccharides/*immunology/pharmacology; Lung Neoplasms/metabolism/*pathology; Membrane Proteins/antagonists & inhibitors/*metabolism; Mice; Nitrobenzenes/pharmacology; Receptor, Epidermal Growth Factor/antagonists & inhibitors/*metabolism; Sulfonamides/pharmacology; Toll-Like Receptor 4/antagonists & inhibitors/metabolism
External Publication Status:published
Document Type:Article
Communicated by:Svea Hümmer
Affiliations:MPI für physiologische und klinische Forschung
Identifiers:ISSN:1432-0851 (Electronic) 0340-7004 (Linking) %R 10.1007/s00262-012-1341-2
URL:http://www.ncbi.nlm.nih.gov/pubmed/22923191
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