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          Institute: MPI für Entwicklungsbiologie     Collection: Abteilung 3 (bis 2014)- Genetics (C. Nüsslein-Volhard)     Display Documents

ID: 683607.0, MPI für Entwicklungsbiologie / Abteilung 3 (bis 2014)- Genetics (C. Nüsslein-Volhard)
Slc45a2 and V-ATPase are regulators of melanosomal pH homeostasis in zebrafish, providing a mechanism for human pigment evolution and disease
Authors:Dooley, C. M.; Schwarz, H.; Mueller, K. P.; Mongera, A.; Konantz, M.; Neuhauss, S. C.; Nusslein-Volhard, C.; Geisler, R.
Date of Publication (YYYY-MM-DD):2013-03
Title of Journal:Pigment Cell & Melanoma Research
Journal Abbrev.:Pigment Cell Melanoma Res
Issue / Number:2
Start Page:205
End Page:217
Sequence Number of Article:23205854
Review Status:Internal review
Audience:Experts Only
Abstract / Description:We present here the positional cloning of the Danio rerio albino mutant and show that the affected gene encodes Slc45a2. The human orthologous gene has previously been shown to be involved in human skin color variation, and mutations therein have been implicated in the disease OCA4. Through ultrastructural analysis of the melanosomes in albino alleles as well as the tyrosinase-deficient mutant sandy, we add new insights into the role of Slc45a2 in the production of melanin. To gain further understanding of the role of Slc45a2 and its possible interactions with other proteins involved in melanization, we further analyzed the role of the V-ATPase as a melanosomal acidifier. We show that it is possible to rescue the melanization potential of the albino melanosomes through genetic and chemical inhibition of V-ATPase, thereby increasing internal melanosome pH.
Free Keywords:Alleles; Amino Acid Sequence; Animals; *Biological Evolution; Cloning, Molecular; *Homeostasis/drug effects; Humans; Hydrogen-Ion Concentration/drug effects; Melanocytes/metabolism/pathology; Melanophores/drug effects/metabolism; Melanosomes/drug effects/*metabolism/ultrastructure; Membrane Transport Proteins/chemistry/*metabolism; Models, Biological; Molecular Sequence Data; Monophenol Monooxygenase/metabolism; Morpholinos/pharmacology; Mutation/genetics; Neural Crest/drug effects/metabolism/pathology; Organ Specificity/drug effects; *Pigmentation/drug effects; Protein Structure, Tertiary; Protein Subunits/chemistry/metabolism; Retina/drug effects/metabolism/pathology; Vacuolar Proton-Translocating ATPases/antagonists & inhibitors/*metabolism; Visual Acuity/drug effects; Zebrafish/*metabolism; Zebrafish Proteins/chemistry/*metabolism
External Publication Status:published
Document Type:Article
Communicated by:MPI für Entwickungsbiologie
Affiliations:MPI für Entwicklungsbiologie/Abteilung 3 - Genetik (Christiane Nüsslein-Volhard)
Identifiers:ISSN:1755-148X (Electronic) 1755-1471 (Linking) %R 10.1... [ID No:1]
URL:http://www.ncbi.nlm.nih.gov/pubmed/23205854 [ID No:2]
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