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          Institute: MPI für molekulare Zellbiologie und Genetik     Collection: MPI-CBG Publications 2013 (arch)     Display Documents



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ID: 688491.0, MPI für molekulare Zellbiologie und Genetik / MPI-CBG Publications 2013 (arch)
Cycling of the signaling protein phospholipase D through cilia requires the BBSome only for the export phase.
Authors:Lechtreck, Karl F; Brown, Jason M; Sampaio, Julio; Craft, Julie M; Shevchenko, Andrej; Evans, James E; Witman, George B
Date of Publication (YYYY-MM-DD):2013
Title of Journal:The Journal of Cell Biology
Volume:201
Issue / Number:2
Start Page:249
End Page:261
Copyright:not available
Audience:Experts Only
Intended Educational Use:No
Abstract / Description:The BBSome is a complex of seven proteins, including BBS4, that is cycled through cilia by intraflagellar transport (IFT). Previous work has shown that the membrane-associated signaling protein phospholipase D (PLD) accumulates abnormally in cilia of Chlamydomonas reinhardtii bbs mutants. Here we show that PLD is a component of wild-type cilia but is enriched ∼150-fold in bbs4 cilia; this accumulation occurs progressively over time and results in altered ciliary lipid composition. When wild-type BBSomes were introduced into bbs cells, PLD was rapidly removed from the mutant cilia, indicating the presence of an efficient BBSome-dependent mechanism for exporting ciliary PLD. This export requires retrograde IFT. Importantly, entry of PLD into cilia is BBSome and IFT independent. Therefore, the BBSome is required only for the export phase of a process that continuously cycles PLD through cilia. Another protein, carbonic anhydrase 6, is initially imported normally into bbs4 cilia but lost with time, suggesting that its loss is a secondary effect of BBSome deficiency.
External Publication Status:published
Document Type:Article
Version Comment:Automatic journal name synchronization
Communicated by:Lib
Affiliations:MPI für molekulare Zellbiologie und Genetik
Identifiers:LOCALID:5595
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