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          Institute: MPI für molekulare Zellbiologie und Genetik     Collection: MPI-CBG Publications 2013 (arch)     Display Documents



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ID: 688518.0, MPI für molekulare Zellbiologie und Genetik / MPI-CBG Publications 2013 (arch)
HOT1 is a mammalian direct telomere repeat-binding protein contributing to telomerase recruitment.
Authors:Kappei, Dennis; Butter, Falk; Benda, Christian; Scheibe, Marion; Draskovic, Irena; Stevense, Michelle; Novo, Clara Lopes; Basquin, Claire; Araki, Masatake; Araki, Kimi; Krastev, Dragomir; Kittler, Ralf; Jessberger, Rolf; Londoño-Vallejo, J Arturo; Mann, Matthias; Buchholz, Frank
Date of Publication (YYYY-MM-DD):2013
Title of Journal:The EMBO Journal
Volume:32
Issue / Number:12
Start Page:1681
End Page:1701
Copyright:not available
Review Status:Internal review
Audience:Experts Only
Intended Educational Use:No
Abstract / Description:Telomeres are repetitive DNA structures that, together with the shelterin and the CST complex, protect the ends of chromosomes. Telomere shortening is mitigated in stem and cancer cells through the de novo addition of telomeric repeats by telomerase. Telomere elongation requires the delivery of the telomerase complex to telomeres through a not yet fully understood mechanism. Factors promoting telomerase-telomere interaction are expected to directly bind telomeres and physically interact with the telomerase complex. In search for such a factor we carried out a SILAC-based DNA-protein interaction screen and identified HMBOX1, hereafter referred to as homeobox telomere-binding protein 1 (HOT1). HOT1 directly and specifically binds double-stranded telomere repeats, with the in vivo association correlating with binding to actively processed telomeres. Depletion and overexpression experiments classify HOT1 as a positive regulator of telomere length. Furthermore, immunoprecipitation and cell fractionation analyses show that HOT1 associates with the active telomerase complex and promotes chromatin association of telomerase. Collectively, these findings suggest that HOT1 supports telomerase-dependent telomere elongation.
External Publication Status:published
Document Type:Article
Version Comment:Automatic journal name synchronization
Communicated by:Lib
Affiliations:MPI für molekulare Zellbiologie und Genetik
Identifiers:LOCALID:5685
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