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          Institute: MPI für molekulare Zellbiologie und Genetik     Collection: MPI-CBG Publications 2013 (arch)     Display Documents



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ID: 688534.0, MPI für molekulare Zellbiologie und Genetik / MPI-CBG Publications 2013 (arch)
Aurora B and Kif2A control microtubule length for assembly of a functional central spindle during anaphase.
Authors:Uehara, Ryota; Tsukada, Yuki; Kamasaki, Tomoko; Poser, Ina; Yoda, Kinya; Gerlich, Daniel W; Goshima, Gohta
Date of Publication (YYYY-MM-DD):2013
Title of Journal:The Journal of Cell Biology
Volume:202
Issue / Number:4
Start Page:623
End Page:636
Copyright:not available
Audience:Experts Only
Intended Educational Use:No
Abstract / Description:The central spindle is built during anaphase by coupling antiparallel microtubules (MTs) at a central overlap zone, which provides a signaling scaffold for the regulation of cytokinesis. The mechanisms underlying central spindle morphogenesis are still poorly understood. In this paper, we show that the MT depolymerase Kif2A controls the length and alignment of central spindle MTs through depolymerization at their minus ends. The distribution of Kif2A was limited to the distal ends of the central spindle through Aurora B-dependent phosphorylation and exclusion from the spindle midzone. Overactivation or inhibition of Kif2A affected interchromosomal MT length and disorganized the central spindle, resulting in uncoordinated cell division. Experimental data and model simulations suggest that the steady-state length of the central spindle and its symmetric position between segregating chromosomes are predominantly determined by the Aurora B activity gradient. On the basis of these results, we propose a robust self-organization mechanism for central spindle formation.
External Publication Status:published
Document Type:Article
Version Comment:Automatic journal name synchronization
Communicated by:Lib
Affiliations:MPI für molekulare Zellbiologie und Genetik
Identifiers:LOCALID:5533
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