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          Institute: MPI für molekulare Zellbiologie und Genetik     Collection: MPI-CBG Publications 2013 (arch)     Display Documents



ID: 688555.0, MPI für molekulare Zellbiologie und Genetik / MPI-CBG Publications 2013 (arch)
The human kinesin-14 HSET tracks the tips of growing microtubules in vitro.
Authors:Braun, Marcus; Lansky, Zdenek; Bajer, Seweryn; Fink, Gero; Kasprzak, Andrzej A; Diez, Stefan
Date of Publication (YYYY-MM-DD):2013
Title of Journal:Cytoskeleton (Hoboken, N.J.)
Volume:70
Issue / Number:9
Start Page:515
End Page:521
Copyright:not available
Audience:Experts Only
Intended Educational Use:No
Abstract / Description:Tip-tracking of kinesin-14 motor proteins is believed to be crucial for the assembly and maintenance of dynamic microtubule arrays. However, in contrast to other members of the kinesin-14 family, H. sapiens kinesin-14 HSET has so far never been observed to be prominently located at microtubule plus ends. Here, using an in vitro microtubule dynamics reconstitution assay we observe tip-tracking of GFP-HSET in the presence of H. sapiens EB1 (hsEB1). Tip-tracking depended on the SxIP-like motif in HSET as well as on the EB homology domain in hsEB1. D. melanogaster Ncd and S. pombe Klp2 tip-tracking reconstitution assays accompanied by kinesin-14 amino acid sequence comparisons suggest that SxIP-like motif mediated tip-tracking dependent on EB family proteins is conserved in the kinesin-14 family of molecular motors.
External Publication Status:published
Document Type:Article
Communicated by:Lib
Affiliations:MPI für molekulare Zellbiologie und Genetik
Identifiers:LOCALID:5559
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