Home News About Us Contact Contributors Disclaimer Privacy Policy Help FAQ

Home
Search
Quick Search
Advanced
Fulltext
Browse
Collections
Persons
My eDoc
Session History
Login
Name:
Password:
Documentation
Help
Support Wiki
Direct access to
document ID:


          Institute: MPI für molekulare Zellbiologie und Genetik     Collection: MPI-CBG Publications 2013 (arch)     Display Documents



  history
ID: 688556.0, MPI für molekulare Zellbiologie und Genetik / MPI-CBG Publications 2013 (arch)
XMAP215 activity sets spindle length by controlling the total mass of spindle microtubules
Authors:Reber, Simone; Baumgart, Johannes; Widlund, Per; Pozniakovsky, Andrei I.; Howard, Jonathon; Hyman, Anthony; Jülicher, Frank
Date of Publication (YYYY-MM-DD):2013
Title of Journal:Nature Cell Biology
Volume:15
Issue / Number:9
Start Page:1116
End Page:1122
Copyright:not available
Audience:Experts Only
Intended Educational Use:No
Abstract / Description:Metaphase spindles are microtubule-based structures that use a multitude of proteins to modulate their morphology and function. Today, we understand many details of microtubule assembly, the role of microtubule-associated proteins, and the action of molecular motors. Ultimately, the challenge remains to understand how the collective behaviour of these nanometre-scale processes gives rise to a properly sized spindle on the micrometre scale. By systematically engineering the enzymatic activity of XMAP215, a processive microtubule polymerase, we show that Xenopus laevis spindle length increases linearly with microtubule growth velocity, whereas other parameters of spindle organization, such as microtubule density, lifetime and spindle shape, remain constant. We further show that mass balance can be used to link the global property of spindle size to individual microtubule dynamic parameters. We propose that spindle length is set by a balance of non-uniform nucleation and global microtubule disassembly in a liquid-crystal-like arrangement of microtubules.
External Publication Status:published
Document Type:Article
Version Comment:Automatic journal name synchronization
Communicated by:Lib
Affiliations:MPI für molekulare Zellbiologie und Genetik
Identifiers:LOCALID:5503
The scope and number of records on eDoc is subject to the collection policies defined by each institute - see "info" button in the collection browse view.