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          Institute: MPI für molekulare Zellbiologie und Genetik     Collection: MPI-CBG Publications 2013 (arch)     Display Documents

ID: 688605.0, MPI für molekulare Zellbiologie und Genetik / MPI-CBG Publications 2013 (arch)
The human cap-binding complex is functionally connected to the nuclear RNA exosome.
Authors:Andersen, Peter Refsing; Domanski, Michal; Kristiansen, Maiken S; Storvall, Helena; Ntini, Evgenia; Verheggen, Celine; Schein, Aleks; Bunkenborg, Jakob; Poser, Ina; Hallais, Marie; Sandberg, Rickard; Hyman, Anthony; Lacava, John; Rout, Michael P; Andersen, Jens S; Bertrand, Edouard; Jensen, Torben Heick
Date of Publication (YYYY-MM-DD):2013
Title of Journal:Nature Structural & Molecular Biology
Issue / Number:12
Start Page:1367
End Page:1376
Copyright:not available
Audience:Experts Only
Intended Educational Use:No
Abstract / Description:Nuclear processing and quality control of eukaryotic RNA is mediated by the RNA exosome, which is regulated by accessory factors. However, the mechanism of exosome recruitment to its ribonucleoprotein (RNP) targets remains poorly understood. Here we report a physical link between the human exosome and the cap-binding complex (CBC). The CBC associates with the ARS2 protein to form CBC-ARS2 (CBCA) and then further connects, together with the ZC3H18 protein, to the nuclear exosome targeting (NEXT) complex, thus forming CBC-NEXT (CBCN). RNA immunoprecipitation using CBCN factors as well as the analysis of combinatorial depletion of CBCN and exosome components underscore the functional relevance of CBC-exosome bridging at the level of target RNA. Specifically, CBCA suppresses read-through products of several RNA families by promoting their transcriptional termination. We suggest that the RNP 5' cap links transcription termination to exosomal RNA degradation through CBCN.
External Publication Status:published
Document Type:Article
Version Comment:Automatic journal name synchronization
Communicated by:Lib
Affiliations:MPI für molekulare Zellbiologie und Genetik
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