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Home Search Quick Search Advanced Fulltext Browse Collections Persons My eDoc Session History Login Name: Password: Documentation Help Support Wiki Direct access to document ID:	Institute: MPI für molekulare Zellbiologie und Genetik     Collection: MPI-CBG Publications 2013 (arch)     Display Documents   history ID: 688605.0, MPI für molekulare Zellbiologie und Genetik / MPI-CBG Publications 2013 (arch) The human cap-binding complex is functionally connected to the nuclear RNA exosome. Authors: Andersen, Peter Refsing; Domanski, Michal; Kristiansen, Maiken S; Storvall, Helena; Ntini, Evgenia; Verheggen, Celine; Schein, Aleks; Bunkenborg, Jakob; Poser, Ina; Hallais, Marie; Sandberg, Rickard; Hyman, Anthony; Lacava, John; Rout, Michael P; Andersen, Jens S; Bertrand, Edouard; Jensen, Torben Heick Date of Publication (YYYY-MM-DD): 2013 Title of Journal: Nature Structural & Molecular Biology Volume: 20 Issue / Number: 12 Start Page: 1367 End Page: 1376 Copyright: not available Audience: Experts Only Intended Educational Use: No Abstract / Description: Nuclear processing and quality control of eukaryotic RNA is mediated by the RNA exosome, which is regulated by accessory factors. However, the mechanism of exosome recruitment to its ribonucleoprotein (RNP) targets remains poorly understood. Here we report a physical link between the human exosome and the cap-binding complex (CBC). The CBC associates with the ARS2 protein to form CBC-ARS2 (CBCA) and then further connects, together with the ZC3H18 protein, to the nuclear exosome targeting (NEXT) complex, thus forming CBC-NEXT (CBCN). RNA immunoprecipitation using CBCN factors as well as the analysis of combinatorial depletion of CBCN and exosome components underscore the functional relevance of CBC-exosome bridging at the level of target RNA. Specifically, CBCA suppresses read-through products of several RNA families by promoting their transcriptional termination. We suggest that the RNP 5' cap links transcription termination to exosomal RNA degradation through CBCN. External Publication Status: published Document Type: Article Version Comment: Automatic journal name synchronization Communicated by: Lib Affiliations: MPI für molekulare Zellbiologie und Genetik Identifiers: LOCALID:5558 The scope and number of records on eDoc is subject to the collection policies defined by each institute - see "info" button in the collection browse view.