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          Institute: MPI für Entwicklungsbiologie     Collection: Abteilung 1 - Protein Evolution (A. Lupas)     Display Documents



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ID: 695269.0, MPI für Entwicklungsbiologie / Abteilung 1 - Protein Evolution (A. Lupas)
A trimeric lipoprotein assists in trimeric autotransporter biogenesis in enterobacteria
Authors:Grin, I.; Hartmann, M. D.; Sauer, G.; Hernandez Alvarez, B.; Schutz, M.; Wagner, S.; Madlung, J.; Macek, B.; Felipe-Lopez, A.; Hensel, M.; Lupas, A.; Linke, D.
Language:English
Date of Publication (YYYY-MM-DD):2014-03-14
Title of Journal:The Journal of Biological Chemistry
Journal Abbrev.:J Biol Chem
Volume:289
Issue / Number:11
Start Page:7388
End Page:7398
Sequence Number of Article:24369174
Review Status:Internal review
Audience:Experts Only
Abstract / Description:Trimeric autotransporter adhesins (TAAs) are important virulence factors of many Gram-negative bacterial pathogens. TAAs form fibrous, adhesive structures on the bacterial cell surface. Their N-terminal extracellular domains are exported through a C-terminal membrane pore; the insertion of the pore domain into the bacterial outer membrane follows the rules of beta-barrel transmembrane protein biogenesis and is dependent on the essential Bam complex. We have recently described the full fiber structure of SadA, a TAA of unknown function in Salmonella and other enterobacteria. In this work, we describe the structure and function of SadB, a small inner membrane lipoprotein. The sadB gene is located in an operon with sadA; orthologous operons are only found in enterobacteria, whereas other TAAs are not typically associated with lipoproteins. Strikingly, SadB is also a trimer, and its co-expression with SadA has a direct influence on SadA structural integrity. This is the first report of a specific export factor of a TAA, suggesting that at least in some cases TAA autotransport is assisted by additional periplasmic proteins.
Free Keywords:Autotransport, Bacterial Adhesion, Bacterial Pathogenesis, Crystal Structure, Enterobacteria, Gram-negative Bacteria, Lipoprotein, Membrane Insertion, Membrane Trafficking
External Publication Status:published
Document Type:Article
Affiliations:MPI für Entwicklungsbiologie/Abteilung 1 - Proteinevolution (Andrei Lupas)
Identifiers:ISSN:1083-351X (Electronic) 0021-9258 (Linking) %R 10.1... [ID No:1]
URL:http://www.ncbi.nlm.nih.gov/pubmed/24369174 [ID No:2]
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