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          Institute: MPI für bioanorganische Chemie     Collection: MPI für bioanorganische Chemie     Display Documents



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ID: 697707.0, MPI für bioanorganische Chemie / MPI für bioanorganische Chemie
A cyanobacterial light activated adenylyl cyclase partially restores development of a Dictyostelium discoideum, adenylyl cyclase a null mutant
Authors:Chen, Zhi-Hui; Raffelberg, Sarah; Losi, Aba; Schaap, Pauline; Gärtner, Wolfgang
Language:English
Date of Publication (YYYY-MM-DD):2014
Title of Journal:Journal of Biotechnology
Journal Abbrev.:J. Biotechnol.
Volume:191
Issue / Number:Sp. Iss. SI
Start Page:246
End Page:249
Review Status:Peer-review
Audience:Experts Only
Abstract / Description:A light-regulated adenylyl cyclase, mPAC, was previously identified from the cyanobacterium Microcoleus chthonoplastes PCC7420. MPAC consists of a flavin-based blue light-sensing LOV domain and a catalytic domain. In this work, we expressed mPAC in an adenylate cyclase A null mutant (aca-) of the eukaryote Dictyostelium discoideum and tested to what extent light activation of mPAC could restore the cAMP-dependent developmental programme of this organism. Amoebas of Dictyostelium, a well-established model organism, generate and respond to cAMP pulses, which cause them to aggregate and construct fruiting bodies. mPAC was expressed under control of a constitutive actin-15 promoter in D. discoideum and displayed low basal adenylyl cyclase activity in darkness that was about five-fold stimulated by blue light. mPAC expression in aca- cells marginally restored aggregation and fruiting body formation in darkness. However, more and larger fruiting bodies were formed when mPAC expressing cells were incubated in light. Extending former applications of light-regulated AC, these results demonstrate that mPAC can be used to manipulate multicellular development in eukaryotes in a light dependent manner. (C) 2014 Elsevier B.V. All rights reserved
External Publication Status:published
Document Type:Article
Communicated by:N. N.
Affiliations:MPI für bioanorganische Chemie
External Affiliations:[Chen,Z.H.; ; Schaap,P.; ]Univ Dundee, Coll Life Sci, Dundee, Scotland
[Losi,A.;] Univ Parma, Dept Phys & Earth Sci, I-43100 Parma, Italy.
Identifiers:ISI:000345684000031 [ID No:1]
ISSN:0168-1656 [ID No:2]
DOI:10.1016/j.jbiotec.2014.08.008 [ID No:3]
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