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          Institute: MPI für Dynamik und Selbstorganisation     Collection: Nichtlineare Dynamik     Display Documents

ID: 697891.0, MPI für Dynamik und Selbstorganisation / Nichtlineare Dynamik
Uniquantal Release through a Dynamic Fusion Pore Is a Candidate Mechanism of Hair Cell Exocytosis
Authors:Chapochnikov, Nikolai; Takago, Hideki; Huang, Chao-Hua; Pangrsic, Tina; Khimich, Darina; Neef, Jakob; Auge, Elisabeth; Göttfert, Fabian; Hell, Stefan; Wichmann, Carolin; Wolf, Fred; Moser, Tobias
Date of Publication (YYYY-MM-DD):2014-09-04
Title of Journal:Neuron
Issue / Number:6
Start Page:1389
End Page:1403
Review Status:Peer-review
Audience:Experts Only
Abstract / Description:The mechanisms underlying the large amplitudes and heterogeneity of excitatory postsynaptic currents (EPSCs) at inner hair cell (IHC) ribbon synapses are unknown. Based on electrophysiology, electron and superresolution light microscopy, and modeling, we propose that uniquantal exocytosis shaped by a dynamic fusion pore is a candidate neurotransmitter release mechanism in IHCs. Modeling indicated that the extended postsynaptic AMPA receptor clusters enable large uniquantal EPSCs. Recorded multiphasic EPSCs were triggered by similar glutamate amounts as monophasic ones and were consistent with progressive vesicle emptying during pore flickering. The fraction of multiphasic EPSCs decreased in absence of Ca2+ influx and upon application of the Ca2+ channel modulator BayK8644. Our experiments and modeling did not support the two most popular multiquantal release interpretations of EPSC heterogeneity: (1) Ca2+-synchronized exocytosis of multiple vesicles and (2) compound exocytosis fueled by vesicle-to-vesicle fusion. We propose that IHC synapses efficiently use uniquantal glutamate release for achieving high information transmission rates.
External Publication Status:published
Document Type:Article
Communicated by:Folkert Müller-Hoissen
Affiliations:MPI für Dynamik und Selbstorganisation/Nichtlineare Dynamik
External Affiliations:InnerEarLab, Department of Otolaryngology, University Medical Center Göttingen, 37075 Göttingen, Germany
Bernstein Center for Computational Neuroscience, University of Göttingen, 37073 Göttingen, Germany
Department of Rehabilitation for Sensory Functions, Research Institute, National Rehabilitation Center for Persons with Disabilities, Saitama 359-8555, Japan
Collaborative Research Center 889 Cellular Mechanisms of Sensory Processing, 37099 Göttingen, Germany
Molecular Architecture of Synapses Junior Research Group, University Medical Center Göttingen, 37075 Göttingen, Germany
Department of Nanobiophotonics, Max Planck Institute for Biophysical Chemistry, 37077 Göttingen, Germany
Center for Nanoscale Microscopy and Molecular Physiology of the Brain, University of Göttingen, 37073 Göttingen, Germany
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