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          Institute: MPI für molekulare Zellbiologie und Genetik     Collection: MPI-CBG Publications 2014 (arch)     Display Documents

ID: 705661.0, MPI für molekulare Zellbiologie und Genetik / MPI-CBG Publications 2014 (arch)
Galectin-3 drives glycosphingolipid-dependent biogenesis of clathrin-independent carriers.
Authors:Lakshminarayan, Ramya; Wunder, Christian; Becken, Ulrike; Howes, Mark T; Benzing, Carola; Arumugam, Senthil; Sales, Susanne; Ariotti, Nicholas; Chambon, Valérie; Lamaze, Christophe; Loew, Damarys; Shevchenko, Andrej; Gaus, Katharina; Parton, Robert G.; Johannes, Ludger
Date of Publication (YYYY-MM-DD):2014
Title of Journal:Nature Cell Biology
Issue / Number:6
Start Page:595
End Page:606
Copyright:not available
Audience:Experts Only
Intended Educational Use:No
Abstract / Description:Several cell surface molecules including signalling receptors are internalized by clathrin-independent endocytosis. How this process is initiated, how cargo proteins are sorted and membranes are bent remains unknown. Here, we found that a carbohydrate-binding protein, galectin-3 (Gal3), triggered the glycosphingolipid (GSL)-dependent biogenesis of a morphologically distinct class of endocytic structures, termed clathrin-independent carriers (CLICs). Super-resolution and reconstitution studies showed that Gal3 required GSLs for clustering and membrane bending. Gal3 interacted with a defined set of cargo proteins. Cellular uptake of the CLIC cargo CD44 was dependent on Gal3, GSLs and branched N-glycosylation. Endocytosis of β1-integrin was also reliant on Gal3. Analysis of different galectins revealed a distinct profile of cargoes and uptake structures, suggesting the existence of different CLIC populations. We conclude that Gal3 functionally integrates carbohydrate specificity on cargo proteins with the capacity of GSLs to drive clathrin-independent plasma membrane bending as a first step of CLIC biogenesis.
External Publication Status:published
Document Type:Article
Version Comment:Automatic journal name synchronization
Communicated by:thuem
Affiliations:MPI für molekulare Zellbiologie und Genetik
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