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          Institute: MPI für molekulare Zellbiologie und Genetik     Collection: MPI-CBG Publications 2014 (arch)     Display Documents



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ID: 705685.0, MPI für molekulare Zellbiologie und Genetik / MPI-CBG Publications 2014 (arch)
High-resolution reconstruction of the beating zebrafish heart.
Authors:Mickoleit, Michaela; Schmid, Benjamin; Weber, Michael; Fahrbach, Florian; Hombach, Sonja; Reischauer, Sven; Huisken, Jan
Date of Publication (YYYY-MM-DD):2014
Title of Journal:Nature Methods
Volume:11
Issue / Number:9
Start Page:919
End Page:922
Copyright:not available
Audience:Experts Only
Intended Educational Use:No
Abstract / Description:The heart's continuous motion makes it difficult to capture high-resolution images of this organ in vivo. We developed tools based on high-speed selective plane illumination microscopy (SPIM), offering pristine views into the beating zebrafish heart. We captured three-dimensional cardiac dynamics with postacquisition synchronization of multiview movie stacks, obtained static high-resolution reconstructions by briefly stopping the heart with optogenetics and resolved nonperiodic phenomena by high-speed volume scanning with a liquid lens.
External Publication Status:published
Document Type:Article
Version Comment:Automatic journal name synchronization
Communicated by:thuem
Affiliations:MPI für molekulare Zellbiologie und Genetik
Identifiers:LOCALID:5826
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