Home News About Us Contact Contributors Disclaimer Privacy Policy Help FAQ

Home
Search
Quick Search
Advanced
Fulltext
Browse
Collections
Persons
My eDoc
Session History
Login
Name:
Password:
Documentation
Help
Support Wiki
Direct access to
document ID:


          Institute: MPI für molekulare Zellbiologie und Genetik     Collection: MPI-CBG Publications 2014 (arch)     Display Documents



ID: 705697.0, MPI für molekulare Zellbiologie und Genetik / MPI-CBG Publications 2014 (arch)
Genome-wide RNA Tomography in the Zebrafish Embryo.
Authors:Junker, Jan Philipp; Noël, Emily S.; Guryev, Victor; Peterson, Kevin A; Shah, Gopi; Huisken, Jan; McMahon, Andrew P.; Berezikov, Eugene; Bakkers, Jeroen; Oudenaarden, Alexander van
Date of Publication (YYYY-MM-DD):2014
Title of Journal:Cell
Volume:159
Issue / Number:3
Start Page:662
End Page:675
Copyright:not available
Audience:Experts Only
Intended Educational Use:No
Abstract / Description:Advancing our understanding of embryonic development is heavily dependent on identification of novel pathways or regulators. Although genome-wide techniques such as RNA sequencing are ideally suited for discovering novel candidate genes, they are unable to yield spatially resolved information in embryos or tissues. Microscopy-based approaches, using in situ hybridization, for example, can provide spatial information about gene expression, but are limited to analyzing one or a few genes at a time. Here, we present a method where we combine traditional histological techniques with low-input RNA sequencing and mathematical image reconstruction to generate a high-resolution genome-wide 3D atlas of gene expression in the zebrafish embryo at three developmental stages. Importantly, our technique enables searching for genes that are expressed in specific spatial patterns without manual image annotation. We envision broad applicability of RNA tomography as an accurate and sensitive approach for spatially resolved transcriptomics in whole embryos and dissected organs.
External Publication Status:published
Document Type:Article
Communicated by:thuem
Affiliations:MPI für molekulare Zellbiologie und Genetik
Identifiers:LOCALID:5851
The scope and number of records on eDoc is subject to the collection policies defined by each institute - see "info" button in the collection browse view.