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          Institute: MPI für molekulare Zellbiologie und Genetik     Collection: MPI-CBG Publications 2014 (arch)     Display Documents

ID: 705700.0, MPI für molekulare Zellbiologie und Genetik / MPI-CBG Publications 2014 (arch)
Liver-restricted repin1 deficiency improves whole-body insulin sensitivity, alters lipid metabolism, and causes secondary changes in adipose tissue in mice.
Authors:Kern, Matthias; Kosacka, Joanna; Hesselbarth, Nico; Brückner, Julia; Heiker, John T; Flehmig, Gesine; Klöting, Ingrid; Kovacs, Peter; Matz-Soja, Madlen; Gebhardt, Rolf; Krohn, Knut; Sales, Susanne; Abshagen, Kerstin; Shevchenko, Andrej; Stumvoll, Michael; Blüher, Matthias; Klöting, Nora
Date of Publication (YYYY-MM-DD):2014
Title of Journal:Diabetes
Issue / Number:10
Start Page:3295
End Page:3309
Copyright:not available
Audience:Experts Only
Intended Educational Use:No
Abstract / Description:Replication initiator 1 (Repin1) is a zinc finger protein highly expressed in liver and adipose tissue and maps within a quantitative trait locus (QTL) for body weight and triglyceride (TG) levels in the rat. The QTL has further been supported as a susceptibility locus for dyslipidemia and related metabolic disorders in congenic and subcongenic rat strains. Here, we elucidated the role of Repin1 in lipid metabolism in vivo. We generated a liver-specific Repin1 knockout mouse (LRep1(-/-)) and systematically characterized the consequences of Repin1 deficiency in the liver on body weight, glucose and lipid metabolism, liver lipid patterns, and protein/mRNA expression. Hyperinsulinemic-euglycemic clamp studies revealed significantly improved whole-body insulin sensitivity in LRep1(-/-) mice, which may be due to significantly lower TG content in the liver. Repin1 deficiency causes significant changes in potential downstream target molecules including Cd36, Pparγ, Glut2 protein, Akt phosphorylation, and lipocalin2, Vamp4, and Snap23 mRNA expression. Mice with hepatic deletion of Repin1 display secondary changes in adipose tissue function, which may be mediated by altered hepatic expression of lipocalin2 or chemerin. Our findings indicate that Repin1 plays a role in insulin sensitivity and lipid metabolism by regulating key genes of glucose and lipid metabolism.
External Publication Status:published
Document Type:Article
Communicated by:thuem
Affiliations:MPI für molekulare Zellbiologie und Genetik
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