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          Institute: MPI für molekulare Zellbiologie und Genetik     Collection: MPI-CBG Publications 2014 (arch)     Display Documents



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ID: 705749.0, MPI für molekulare Zellbiologie und Genetik / MPI-CBG Publications 2014 (arch)
The segmentation of microtubules in electron tomograms using Amira.
Authors:Redemann, Stefanie; Weber, Britta; Möller, Marit; Verbavatz, Jean-Marc; Hyman, Anthony; Baum, Daniel; Prohaska, Sonja J; Müller-Reichert, Thomas
Date of Publication (YYYY-MM-DD):2014
Title of Journal:Methods in Molecular Biology (Clifton, N.J.)
Volume:1136
Start Page:261
End Page:278
Copyright:not available
Audience:Experts Only
Intended Educational Use:No
Abstract / Description:The development of automatic tools for the three-dimensional reconstruction of the microtubule cytoskeleton is crucial for large-scale analysis of mitotic spindles. Recently, we have published a method for the semiautomatic tracing of microtubules based on 3D template matching (Weber et al., J Struct Biol 178:129-138, 2012). Here, we give step-by-step instructions for the automatic tracing of microtubules emanating from centrosomes in the early mitotic Caenorhabditis elegans embryo. This approach, integrated in the visualization and data analysis software Amira, is applicable to tomographic data sets from other model systems.
External Publication Status:published
Document Type:Article
Version Comment:Automatic journal name synchronization
Communicated by:thuem
Affiliations:MPI für molekulare Zellbiologie und Genetik
Identifiers:LOCALID:6024
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