Home News About Us Contact Contributors Disclaimer Privacy Policy Help FAQ

Home
Search
Quick Search
Advanced
Fulltext
Browse
Collections
Persons
My eDoc
Session History
Login
Name:
Password:
Documentation
Help
Support Wiki
Direct access to
document ID:


          Institute: MPI für Entwicklungsbiologie     Collection: Abteilungsunabhängige Arbeitsgruppen     Display Documents



  history
ID: 706366.0, MPI für Entwicklungsbiologie / Abteilungsunabhängige Arbeitsgruppen
Characterization of genetic diversity in the nematode Pristionchus pacificus from population-scale resequencing data
Authors:Rodelsperger, C.; Neher, R. A.; Weller, A. M.; Eberhardt, G.; Witte, H.; Mayer, W. E.; Dieterich, C.; Sommer, R. J.
Date of Publication (YYYY-MM-DD):2014-04
Title of Journal:Genetics
Journal Abbrev.:Genetics
Volume:196
Issue / Number:4
Start Page:1153
End Page:1165
Sequence Number of Article:24443445
Review Status:Internal review
Audience:Experts Only
Abstract / Description:The hermaphroditic nematode Pristionchus pacificus is an established model system for comparative studies with Caenorhabditis elegans in developmental biology, ecology, and population genetics. In this study, we present whole-genome sequencing data of 104 P. pacificus strains and the draft assembly of the obligate outcrossing sister species P. exspectatus. We characterize genetic diversity within P. pacificus and investigate the population genetic processes shaping this diversity. P. pacificus is 10 times more diverse than C. elegans and exhibits substantial population structure that allows us to probe its evolution on multiple timescales. Consistent with reduced effective recombination in this self-fertilizing species, we find haplotype blocks that span several megabases. Using the P. exspectatus genome as an outgroup, we polarized variation in P. pacificus and found a site frequency spectrum (SFS) that decays more rapidly than expected in neutral models. The SFS at putatively neutral sites is U shaped, which is a characteristic feature of pervasive linked selection. Based on the additional findings (i) that the majority of nonsynonymous variation is eliminated over timescales on the order of the separation between clades, (ii) that diversity is reduced in gene-rich regions, and (iii) that highly differentiated clades show very similar patterns of diversity, we conclude that purifying selection on many mutations with weak effects is a major force shaping genetic diversity in P. pacificus.
Free Keywords:Animals; Evolution, Molecular; Genetic Variation; *Genome, Helminth; Phylogeography; Rhabditida/*classification/*genetics; Selection, Genetic; Sequence Analysis, DNA
External Publication Status:published
Document Type:Article
Affiliations:MPI für Entwicklungsbiologie/Abteilungsunabhängige Arbeitsgruppen/Arbeitsgruppe Neher
Identifiers:ISSN:1943-2631 (Electronic) 0016-6731 (Linking) %R 10.1... [ID No:1]
URL:http://www.ncbi.nlm.nih.gov/pubmed/24443445 [ID No:2]
The scope and number of records on eDoc is subject to the collection policies defined by each institute - see "info" button in the collection browse view.